Reversal effect of RNA interference targeting FAK on the migration and invasion of cervical cancer cells
FENG Ju;YUAN Zhongfu;QIU Haifeng;Department of Obstetrics and Gynecology, Central Hospital of Jiaozuo Coal Industry (Group) Co., Ltd.;
Objective To investigate the effect and mechanism of FAK on the invasion and migration of cervical cancer cells. Method Cervical cancer cells He La were used in the study, the cells were transfected with RNAi interfering vector, and then were divided as 3 groups: blank control group(untreated cells); negative control group(transfected with FAK-p Genesil-Ctrl vector); interference group(transfected with recombinant plasmid FAK-p Genesil-siRNA). Transwell was applied to determine the migration and invasion of cells, while the expression of FAK, MMP-2, MMP-9 and E-cadherin protein in the cells were detected by Western blot. Result The expression of FAK protein in the interference group was significantly lower than that in the blank control group and the negative control group(P0.05); lower ability of migration and invasion of the cells was observed in interference group than in the blank control group and the negative control group(P0.05); the expression of MMP-2 and MMP-9 in interference group were significantly lower than that of the control group and the negative control group, but the expression of E-cadherin was significantly higher than that of blank control group and negative control group(P0.05). Conclusion The expression of FAK interfered by RNA may lead to decreased ability of invasion, and migration of cervical cancer cells He La, which be related to the expression epithelialmesenchymal transformation-related factors, including MMP-2, MMP-9 and E-cadherin protein.
【CateGory Index】： R737.33