Expression of TSHR mRNA in peripheral blood of patients with papillary thyroid cancer and its relationship with clinical features
ZUO Xiuling;WANG Zhihong;CHEN Haiyan;Department of Endocrinology, the First People's Hospital of Zhengzhou;
Objective To investigate the expression of thyroid stimulating hormone receptor(TSHR) mRNA in the peripheral blood of patients with papillary thyroid cancer(PTC) and its relationship with the clinical features of PTC.Method 50 patients with PTC(study group) and 40 healthy subjects(control group) were included in the comparative analysis. Real-time fluorescence quantitative polymerase chain reaction(PCR) was used to detect the expression of TSHR mRNA in the peripheral blood of two groups of subjects. The association between levels of TSHR mRNA in peripheral blood and clinical features(gender, age, tumor diameter, TNM stage and lymph node metastasis) of patients with PTC was investigated. Result The expression level of TSHR mRNA in peripheral blood of the study group was significantly higher than that of the control group(t=18.192, P0.01). Real-time PCR results showed that the melting curves of TSHR and β-actin gene presented single peak and no primer dimer formation, suggesting that the reaction system was robust,without interference of pollutants. Amplification curves showed good primer quality, non-specific PCR products did not appear. The level of TSHR mRNA in peripheral blood of PTC patients was not significantly associated with gender, age and tumor diameter(P0.05). The TSHR mRNA expression level in patients with stage Ⅲ-Ⅳ disease was significantly higher than that in those with stage Ⅰ-Ⅱ disease(F=14.381, P0.01). TSHR mRNA expression in patients with lymph node metastasis was significantly higher than those without lymph node metastasis(t=4.453, P0.01). Conclusion The expression of TSHR mRNA in peripheral blood of patients with PTC is significantly increased, and is correlated with TNM stage and lymph node metastasis.
【CateGory Index】： R736.1