Expression of KISS1 in patients with metastatic gastric cancer and its association with clinical features and prognosis
YANG An;SHEN Yanfeng;Department of Surgery,Handan Central Hospital;Department of Oncology,Affiliated Cancer Hospital of Hebei University of Engineering;
Objective To investigate the expression of KISS1 in patients with metastatic gastric cancer(MGC) and its association with clinical features and prognosis. Method The specimens of surgical resection of MGC tissues from63 patients with MGC and 50 adjacent normal tissues(distance from resection margin5 cm) among these 63 patients,and 60 specimens of non-metastatic gastric cancer(NMGC) tissues from 60 patients with NMGC were collected. Expressions of KISS1 protein were detected by immunohistochemistry. Result Expression of KISS1 protein was located in the cytoplasm of tumor tissue cells. The positive expression rates of KISS1 protein in MGC, NMGC tissues and adjacent normal tissues were 23.8%(15/63), 43.3%(26/60) and 82.0%(41/50), respectively, the difference was statistically significant(χ2=38.468, P0.05). The positive expression rates of KISS1 protein in MGC and NMGC tissues were significantly lower than that in adjacent normal tissues(P0.05); however, there was no statistical difference in positive expression rate of KISS1 protein between MGC and NMGC tissues(P0.05). The positive expression rate of KISS1 protein was significantly related to general type, vascular thrombosis and the depth of invasion(P0.05). All 63 patients with MGC were followed up, the range of survival time was 4 to 27 months, the median overall survival time was 8 months, 1-year survival rate was 25.4%(16/63). In MGC patients with positive expression of KISS1 protein, median survival time was13 months, 1-year survival rate was 53.3%(8/15); and in MGC patients with negative expression of KISS1 protein, median survival time was 8 months, 1-year survival rate was 16.7%(8/48); there was significant differences in the overall survival(χ2=6.290, P=0.012). Cox regression analysis showed that positive KISS1 protein expression was independent prognostic factors of MGC(HR: 0.442, 95%CI: 0.239-0.818, P=0.009). In NMGC patients with positive expression of KISS1 protein, median tumor-free survival time was 27 months; 1-, 2-and 3-year tumor-free survival rates were 95.7%,60.3% and 43.5%, respectively. In NMGC patients with negative expression of KISS1 protein, median tumor-free survival time was 16 months; 1-, 2-and 3-year tumor-free survival rates were 70.4%, 20.8% and 15.6%, respectively; there was significant difference in the tumor-free survival(χ2=12.176, P0.01). Conclusion The reduced expression of KISS1 protein in gastric cancer tissue is closely associated with invasion, metastasis and poor prognosis of MGC patients, also closely associated with short tumor-free survival in NMGC patients. Expression of KISSl protein is expected to become a marker for predicting the prognosis of patients with gastric cancer.
【CateGory Index】： R735.2