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《Journal of Qingdao University(Medical Sciences)》 2018-03
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PROTECTIVE EFFECT OF ARCTIGENIN AGAINST CONCANAVALIN A-INDUCED ACUTE HEPATITIS IN MICE

FU Xingqin;ZHANG Hui;ZHANG Qingqing;YAO Xiaoying;ZHU Yuzhen;SI Chuanping;Department of Immunology,Medical College of Qingdao University;  
Objective To investigate the protective effect of arctigenin(ATG)against concanavalin A(ConA)-induced acute hepatitis in mice. Methods C57 BL/6 mice were randomly divided into DMSO group,ConA/DMSO group,and ConA/ATG group,with 10 mice in each group.The mice in the DMSO group and the ConA/DMSO group were given intraperitoneal injection of DMSO,and those in the ConA/ATG group were given intraperitoneal injection of the same volume of ATG(30 mg/kg);5 hlater,the mice in the ConA/DMSO group and the ConA/ATG group were given tail vein injection of ConA(20 mg/kg).The mice were sacrificed 12 hlater,and serum and liver tissue samples were collected.An automatic biochemical analyzer was used to measure the serum levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST);HE staining was used to observe liver pathological injury;TUNEL staining was used to measure cell apoptosis;ELISA was used to measure the serum levels of tumor necrosis factor-α(TNF-α)and interferon gamma(IFN-γ);q-PCR was used to measure the mRNA expression of TNF-αand IFN-γin liver tissue;flow cytometry was used to measure myeloid-derived suppressor cells(MDSCs);and the biochemical method was used to measure the expression of malondialdehyde(MDA)and myeloperoxidase(MPO)in liver homogenate. Results Compared with the DMSO group,the ConA/DMSO group had significant increases in the serum levels of ALT and AST(F=125.80 and 94.74,P0.05),significantly greater liver injury,significant increases in hepatocyte apoptosis and the levels of TNF-αand IFN-γin serum and liver tissue(F=17.52 and 68.81,P0.05),a significant reduction in recruitment of MDSCs in the liver,and significant increases in the activities of MDA and MPO(F=13.38 and 14.78,P 0.05).Compared with the ConA/DMSO group,the ConA/ATG group had significant reductions in the serum levels of ALT and AST(F=125.80 and 94.74,P0.05),significant alleviation of liver injury,a significant reduction in hepatocyte apoptosis,significant reductions in the serum level of TNF-αand IFN-γ(F=17.52 and 68.81,P0.05)and the mRNA expression of TNF-αand IFN-γin liver tissue(F=31.55 and 14.64,P0.05),a significant increase in recruitment of MDSCs in the liver,and significant reductions in the activities of MDA and MPO(F=13.38 and 14.78,P0.05). Conclusion ATG has a marked protective effect against ConA-induced acute hepatitis in mice.
【Fund】: 国家自然科学基金资助项目(81671632)
【CateGory Index】: R285.5
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