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《Journal of Qingdao University(Medical Sciences)》 2018-03
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EFFECTS OF GIBBERELLIN ON HUMAN SPERM MOTILITY AND APOPTOSIS IN VITRO AND ITS MECHANISM

XU Chunshuang;ZHOU Yi;JIANG Zhou;ZANG Lili;WANG Li′e;ZOU Shuhua;Reproductive Center of Qingdao Women and Children Hospital Affiliated to Qingdao University;  
Objective To investigate the effects of gibberellin on human sperm motility and apoptosis in vitro and its mechanism. Methods The human sperms with normal physiological function were selected as normal control group using a discontinuous Percoll gradient centrifugation(45%-90%).The high-quality sperms(treatment group)were exposed to G-IVF PLUS medium with different concentrations of gibberellin in vitro.The sperm motility was measured by a sperm quality analyzer at different time points.Flow cytometry was used to determine the level of reactive oxygen species(ROS)and apoptosis.Immunofluorescence was used to measure the expression of proteins related to oxidative stress and apoptosis.The activity of Na~+-K~+-ATP enzyme and Ca~(2+)-ATP enzyme were determined by an ATP enzyme assay kit. Results The sperm motility gradually decreased with time and the increase in gibberellin concentration.Compared with the control group,the treatment group had a significantly higher ROS level(F=21.40,P0.05)and a significantly lower level of superoxide dismutase(F=28.13,P0.05);moreover,the treatment group had significantly higher expression of P53 and BAX(apoptotic proteins)(F=104.54,59.29;P0.05)and significantly lower activity of Na~+-K~+-ATP enzyme and Ca~(2+)-ATP enzyme(F=24.48,18.81;P0.05). Conclusion Gibberellin can reduce sperm motility and promote sperm apoptosis via oxidative stress reaction.
【Fund】: 国家重点研发计划项目(2016YFD0501207)
【CateGory Index】: R99
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