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THE EFFECT OF MERCURY ON CATALYTIC ACTIVITY AND FLUORESCENCE SPECTRA OF HUMAN PLACENTAL ALKALINE PHOSPHATASE

Geng Fangsong, Liu Hongming, Wang Xiuli,Z] et al\ Department of Biochemistry, Qingdao Medical College, Qingdao 266021\;  
Objective \ To study the effect of mercury on catalytic activity and fluorescence spectra of human placental alkaline phosphatase(PLAP) and discuss the molecular mechanism of mercury in inhibiting PLAP.\ X]Methods \ Spectrophotometry was used to study the activity of PLAP under different concentrations of HgCl2. Fluorometry was used to observe the fluorescence spectra. The inhibition type of HgCl2 on PLAP was determined by using the double reciprocal plot.\ Results \ When the concentration of HgCl2 was 0.25mmol/L, PLSP activity and emission intensity were greatly decreased and the emission maximum was blueshifted from 347nm to 340nm. When the concentration of HgCl2 was between 0.25-1.00mmol/L, no obvious changes of PLAP activity, emission intensity and emission maximum were observed. When the concentration of HgCl2 was higher than 1.00mmol/L, the PLAP activity and emission intensity were greatly decreased again along with the decrement of HgCl2 concentration, while the emission maximum kept unchanged, At the concentration of 10mmol/L, some PLAP activity could still be observed. HgCl2 was a mixedcompetitive inhibitor of PLAP and its inhibition constant(ki) was 3.60mmol/L.\ Conclusion \ The PLAP activity was inhibited and its conformation was changed by HgCl2.
【Fund】: 山东省卫生厅科研基金
【CateGory Index】: Q566
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