LOCALIZATION OF LACTATE DEHYDROGENASE C4 (LDH-C4) ON SPERMS BY IMMUNOFLUORESCENCE ASSAY WITH MONOCLONAL ANTIBODIES

FENG SHU-LIN, BEN KUN-LONG, WANG YUN-MEI (Kunming Institute of Zoology, Academia Sinica, Kunming, Yunnan 650223) LIANG ZHI-Guo (National Research Institute for Family Planning, Beijing 100081, China)  

Fourteen monoclonal antibodies (McAbs) were used to determine the localizaton of LDH-C4 on mouse, human and tree shrew sperm by indirect immunofluorescence assay (IIF). McAb PA1 (K021) binds to tail, PA2 (K022), PA4 and PA5 (K023) to tail and midpiece, SM1, SM2 and PG2 to midpiece, SM3 and SM4 to acrosome, SM5 to both acro- some and equatorial of mouse sperm. Similar binding patterns of these McAbs have been found on human and tree shrew sperm, but the binding site for individual McAb may be variable in different species (Table 2). The data from the previous studies were compared with the present results (Table 4). All of the data suggests that LDH-C4 is distributed on multiple sites of sperm and exists in various conformation on different sites of sperm, which is possibly due to the stereonic interaction between LDH-C4 and other molecules. The high homology of the LDH-C4 epitopes from various species tested has also been demonstrated with IIF.

【Fund】： 美国洛氏基金会;; 世界卫生组织人类生殖处;; 国家自然科学基金的资助
【CateGory Index】： Q954

Download(CAJ format) Download(PDF format)

CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.