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《Acta Zoologica Sinica》 1995-01
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ZHENG REN-JIAN, WANG YUN-FEI, SHEN JIE, WANG AI-HUA, ZHOU YONG-ZHI (Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences, Shanghai 200232)  
Restriction endonucleases digestion of kinetoplast DNA minicircle, combined with agarose gel electrophoresis and molecular hybridization to characterize 8 Trypanosoma evansi strains in China was performed. The results presented in restriction enzymes digestion showed that all minicircles of 8 T. evansi strains had identical electrophoretic patterns of restriction fragment with Dde I, Taq I and Sin I. However, the polymorphisms of minor restriction fragment length were observed with Alu I, Hinf I and Mbo I, the minicircles of 6 strains (YNB, ZJB, JSB2, HNB, HBM, GDH) were cleaved once by Hinf I, twice by Mbo I and none by Alu I, and had identical restriction fragment patterns; the minicircles of 2 other strains (XJCA, AHB) were cleaved once by Mbo I, Alu I and twice by Hinf I, When the probe PTK1 was used to hybridize to Hinf I digested fragments, 2 minicircle classes were found with AHB strain. The 8 T. evansi strains could be divided into 2 minicircle types (type A-YNB, ZJB, JSB2, HNB, HBM, GDH and type B-XJCA, AHB) and the type B could be further diveded into type B1 (XJCA)and type B2 (AHB)based on the results in this inves- tigation. The evidence provided by present investigation showed that Chinese T. evansi strains shared kDNA minicircle Sequence homology with each other, but also showed a minor minicircle sequence heterogeneity. It is suggested that low genetic variation occur in Chinese T. evansi, and all strains form a homogeneous species group. However, the above-mentioned minicrcle sequence heterogeneity might be used as a tool for intraspecies classification of T. evansi
【Fund】: 国家自然科学青年基金资助项目,批准编号39000079
【CateGory Index】: Q78
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1 ZHANG Hui~1,SUN Yue-ying~1,LEI Ping~1,ZHANG Wen-juan~1,FU Jing~2(1 Shaanxi Microbiology Rereach Institute,Xi′an 710043,Shaanxi,China;2 College of Life Science,Shaanxi Normal University,Xi′an 710062,Shaanxi, China);Review of studies on mitochondrion-derived organelles and hydrogesome of microrganism[J];Journal of Shaanxi Normal University(Natural Science Edition);2006-S2
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【Secondary References】
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1 HE Mu-rong, ZENG Xiao-fei, CHEN Han-zhong, LI Xiao-xu,TANG Da-yun,BAI Bo,WU Gui-xian(College of Animal Science and Technology, Guangxi University, Nanning,Guangxi,530005,China);Establishment of an Indirect ELISA for Trypanosoma evansi in Water Buffaloes[J];Progress in Veterinary Medicine;2009-03
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