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《Acta Zoologica Sinica》 2002-05
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LOCALIZATION OF 13 NEUROCHEMICAL SUBSTANCES IN THE NERVOUS SYSTEM OF THE CHINESE EARTHWORM (PHERETIMA ASPERGILLUM):AN IMMUNOCYTOCHEMICAL STUDY~*

LUO Zhen Guo ** ZHAO Yue HAN Qing\|Guo ZHANG Xiao Yun (Department of Biotechnology, Shenzhen University,Shenzhen 518060, Guangdong, China)  
About 10 neurochemical substances have been found in the nervous system of Annelids, but no detailed research in this area has been conducted on the Chinese earthworm, P. aspergillum . This research aims to establish if neurochemical substances discovered in other Annelids also exist in P. aspergillum and also if there are any new neurochemical substances in this species. Chinese earthworms were fed with paper pulp and agar to cause them excrete previously ingested earth and sand. They were then deeply anesthetized with 10% ethanol and dissected. The samples were fixed in fixative containing 4% paraformaldehyde and 2% picric acid in 0 01 mol/L PBS (pH 7 2) at 4℃ for 6 hours, the transferred to 30% sucrose PBS until completely infiltrated. Samples were then embedded in OCT compound (USA), quick frozen in liquid nitrogen and sectioned into 20 μm thicknesses using a LEICA CM 1100 cryostat (Pharmacia). The serial sections were stained with antibodies anti adrenocorticotropin hormone (ACTH), anti arginine vasopreesin (AVP), anti calcitonin, anti cholecystokinin (CCK), anti β endorphin (β EP), anti glutamate, anti calbindin D, anti calcitonin gene related peptide (CGRP), anti neuropeptide Y (NPY), anti oxytocin (OT), anti somatostatin (SOM), anti vasoactive intestinal polypeptide (VIP) and anti γ amino butyric acid (GABA) using immunocytochemistry techniques. The immunostained consecutive sections were observed and the positive cells and fibers were examined under a light microscope. It was found that ACTH positive neurons, AVP positive neurons, calcitonin positive neurons containing dense large granules, CCK positive neurons, NPY positive neuron and glutamate positive neurons were stained heavily and distributed densely,but the β EP positive neurons were stained lightly. A few OT positive neurons were detected sparsely distributed in brain tissue. Calbindin D, CGRP, GABA, SOM, VIP immunocytochemical reactions were negative. AVP positive neurons, glutamate positive neurons and NPY positive neurons also were detected in the subpharyngeal ganglion and ventral ganglion.β EP positive neurons, CGRP positive neurons, SOM positive neurons and VIP positive neurons were found in the enteric nervous system. Calcitonin positive epithelial cells, CGRP positive epithelial cells and VIP positive epithelial cells were distributed between negative epithelial cells. The results indicate the presence of not only previously discovered neurochemical substances: ACTH, CCK, CGRP, NPY, VIP, β endorphin also found in the nervous systems of other Annelids, but also new neurochemical substances: calcitonin, glutamate, AVP, OT,SOM. In adition, calcitonin, glutamate, VIP positive epithelial cells were distributed in the digestive system of this species.
【Fund】: 国家自然科学基金项目 (No .3 9770 3 83 )~~
【CateGory Index】: Q45
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【References】
Chinese Journal Full-text Database 2 Hits
1 LUO Zhen Guo ①** ZHAO Yue ② HAN Qing Guo ① (① Department of Biotechnology, Shenzhen University, Shenzhen 518060, China) (② Department of Pharmacology, Guangdong Pharmacology College, Guangzhou 510224, China);IMMUNOCYTOCHEMICAL CHARACTERISTIC OF NSE,NF200,ED1,GFAP and NADPH IN NERVOUS TISSUE OF THE EARTHWORM(PHERETIMA ASPERGILLUM)[J];Acta Zoologica Sinica;2002-06
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【Co-references】
Chinese Journal Full-text Database 9 Hits
1 TCHANG Tso-RUN et TANG CHONG-TI;LA MOUVEMENT DE L'INFRACILIATURE PENDANT LA DIVISION DE PARAMAECIUM AMELIA ET LA CONSIDERATION SUR LA FORMATION DE CYTOSTOME, DE SUTURE ET DE CYTOPYGE[J];Acta Zoologica Sinica;1957-02
2 SHI XINBAI(Harbin Pedagogical College);THE MORPHOLOGY AND MORPHOGENESIS OF THE BUCCAL APPARATUS IN PARAMECIUM AND THEIR PHYLOGENETIC IMPLICATIONS Ⅰ.MORPHOLOGY OF BUCCAL APPARATUS[J];Acta Zoologica Sinica;1980-03
3 SHI XINBAI (Harbin Pedagogical College);THE MORPHOLOGY AND MORPHOGENESIS OF THE BUCCAL APPARATUS OF PARAMECIUM AND THEIR PHYLOGENETIC IMPLICATIONS II.STOMATOGENESIS[J];Acta Zoologica Sinica;1980-04
4 NI ZUMEI SHI LUJI (L. S. SZE) Su QINGXIANG AND SU LIDE(Institute of Cell Biology, Academia Sinica, Shanghai, China);INDIRECT IMMUNOFLOURESCENCE STUDIES ON THE SPINDLE FIBER ATTACHMENTS (SFAs) OF SEVERAL ECONOMICAL ANIMALS[J];Acta Zoologica Sinica;1987-04
5 SUI Shu-Guang CHEN Ying QIU Zi-Jian SHI Xin-Bai (Department of Biology,Harbin Normal University,Harbin 150080,China);STRUCTURE OF ORAL APPARATUS IN STYLONYCHIA MYTILUS[J];Acta Zoologica Sinica;2001-04
6 CHEN Ying QIU Zi Jian SUI Shu Guang SHI Xin Bai (Department of Biology, Harbin Normal University, Harbin 150080, China);Localization of ACPase and its expression by induction in Stylonychia mytilus[J];Acta Zoologica Sinica;2003-02
7 GAO Chongming CHEN Qiushi FENG Lei LI Yingzhen (College of Life Science,Peking University,Beijing,100871);Intermediate-like Filaments in ParameciumA Preliminary Investigation with Immunological Methods[J];Acta Scicentiarum Naturalum Universitis Pekinesis;2001-06
8 CHEN Qiushi\ GAO Chongming (College of Life Science,Peking University,Beijing,100871);A Preliminary Investigation on the Lamin-like Proteins in Paramecium[J];Acta Scicentiarum Naturalum Universitis Pekinesis;2002-02
9 LUO Zhen guo , ZHANG Xiao yun (Life Science Institute, Shenzhen University, Shenzhen\ 518060,China);IMMUNOCYTOCHEMICAL LOCALIZATION OF GLUTAMATE AND METABOTROPIC GLUTAMATE RECEPTOR,mGluR1, mGluR2/3, mGluR4 IN EARTHWORM, P. ASPERGILLUM[J];Acta Anatomica Sinica;2001-04
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 CHEN Jing 1,2,GUAN Ping 1,3,QIU Zi-Jian 11.College of Life and Environmental Sciences,Harbin Normal University,Harbin 150080,China2.College of Life Sciences,Heilongjiang August First Land Reclamation University,Daqing,Heilongjiang 163319,China3.Biological Science & Technology College,Shenyang Agricultural University,Shenyang 10061,China;Intracellular digestive process of food in a fresh water ciliate Stylonychia mytilus(Protozoa,Ciliophora,Hypotrichida)[J];Acta Zoologica Sinica;2008-03
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