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《Acta Zoologica Sinica》 2002-05
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FUSION OF EHRLICH'S ASCITES CELL PLASMA MEMBRANE WITH LIPOSOMES DRIVEN BY TRANSMEMBRANE PRONTON EFFLUX:EFFECTS OF CALCIUM IONS AND LIPOSOME PHOSPHOLIPID COMPOSITION~*

JIAO Xuan Mao LI Qing Huan XU Mei XIONG Jing Wei LI Lin jiang Shao Lan LIU Shu Sen ** (State Key Laboratory of Biomembrane and Membrane Biotechnology,Institute of Zoology, Chinese Academy of Sciences,Beijing 100080,China)SHEN Zi Wei (  
The Effects of Ca 2+ and the surface electric charge density of plasma membranes on membrane fusions driven by proton translocation were studied.It was shown that Ca 2+ could promote the membrane fusion of Ehrlichs ascites cells with liposomes driven by proton translocation across plasma membranes,but the extent of the membrane fusion was not related to the concentration of calcium.The relationship between fusion extent and surface electric charge density of membranes was shown to be closely correlated with each other under various concentrations of Ca 2+ , and liposomes composed of different lipids Ca 2+ 6 mmol/L and PE∶PC∶CL=6∶2∶2,Ca 2+ 6 mmol/L as well as PC∶PE∶CL=6∶2∶2. No correlation was seen with Ca 2+ and PE∶CL=8∶2,Ca 2+ =10 mmol/L and PE∶CL=8∶2.Besides, the effects of the ratio of PE to PC in the liposome on membrane fusion indicated that the extent of membrane fusion of Ehrlichs ascites cells with liposomes decreased with the decrement of PE and increment of PC. This implies that the ability to forme ‘stalk intermediates was one other factor to influence membrane fusion of Ehrlichs ascites cells with liposomes.
【Fund】: 国家自然科学基金资助项目 (No .3 9770 166)~~
【CateGory Index】: Q26
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Xuanmao;Xu Hailou;Liu Shusen(Depertment of Cell and Membrane Biology,Institute of Zoology,Academia Sinica,Beijing 100080)(State Key Laboratory of Biomembrane and Membrane Biotechnology);MEMBRANE FUSION-INDUCED FACTORS CAUSE A DECREASE IN MEMBRANE SURFACE DIELECTRIC CONSTANT[J];ACTA BIOPHYSICA SINICA;1994-03
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