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《Northern Horticulture》 2017-24
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Screening Strains Resistant of Lead and Cadmium and Growth Conditions Optimization

JIN Zhongmin;ZHAO Jingtong;WEN Yuchen;NIE Wenjun;LIU Lijie;College of Life Science and Agriculture and Forestry,Qiqihar University;  
This experiment is based on the soil of Zalong wetland in Heilongjiang Province,using the method of state characteristic observation,physiological and biochemical characteristics and the development analysis of 16 S rDNA sequence systems,the effects of temperature,heavy metal concentration,NaCl concentration,pH,antibiotic type and oxygen on the growth of strain were studied,in order to provide reference for the use of lead-resistant and cadmium strains to control heavy metal pollution.The results showed that a strain resistant to heavy metals was screened and initially identified as Burkholderiasp,the optimum temperature was 30℃and the optimum pH was 7.It could be grown on medium with salt content6%.With the increase of NaCl concentration,the growth of the strain was inhibited.The strain might belong to aerobic bacteria.For other heavy metals,in the low concentration of Cu~(2+) containing medium growth,high concentration inhibition,not resistant to Cr~(6+).The strain could grow in an environment containing different concentrations of antibiotics,high concentrations were inhibited when grew.
【CateGory Index】: X172
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