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《Northern Horticulture》 2017-24
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Cloning and Bioinformatic Analysis of DFR Gene FromIris lactea Pall.var.chinensis(Fisch.)Koidz

HU Juan;AN Shaoya;LIN Zhe;ZHANG Hong;BAO Han;CHEN Ren;Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China,Ningxia University;School of Life Science,Ningxia University;  
Iris lactea Pall.var.chinensis(Fisch.)Koidz petals were used as material for transcriptome sequencing.According to the annotation data,a key gene encodes dihydvroflavonol-4-reductase(DFR)in its anthocyanin biosynthesis was cloned by using PCR amplification and was charactered using bioinformatic analysis.The full-length of DFR gene of I.lactea var.chinensis consisted of 1 427 bp encoding aprotein with 357 amino acid residues(designated as IlDFR,accession No.KY907171).Based on the comparison of the deduced amino acid sequence,IlDFR shared high identity with other plant DFRs,and had high homology with congeneric Iris hollandica DFR.IlDFR had a predicted molecular mass of 39.99 kDa and an isoelectric point of 5.89.It mainly composed ofα-helixes andβ-sheets,and was considered as an acidic,hydrophilic,no signal peptide and labile protein that located in the cytoplasm.IlDFR had a ‘VTGASGYVGSWLVMKLLRDGY'amino acid sequence that was similar to the NADPH binding domain which was a typical conserved sequence in most of other plant DFRs,but differenced in 4 amino acid residues.
【Fund】: 2013年度宁夏回族自治区科技支撑计划资助项目
【CateGory Index】: S682.19
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