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《Northern Horticulture》 2017-24
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Classification and Identifition of Mycorrhizal Fungi of Paphiopedilum micranthum

TIAN Fan;LIAO Xiaofeng;WANG Lianhui;ZHU Guosheng;GUI Yang;BAI Xinxiang;Guizhou Forestry Academy;Institute of Mountain Resources,Guizhou Academy of Sciences;Institute of Morden Chinese Medical Materials,Guizhou Academy of Agricaltural Sciences;College of Forestry,Guizhou University;  
Classification and identification of mycorrhizal were associated with Paphiopedilum micranthum,the method of morphological was combined with the ITS gene fragments were PCR amplified and sequenced,the rDNA ITS regions were analyzed by means of the software of NCBI BLAST.The results showed that the 29 strains with different micro-and macro-morphological.Secondly,rDNA-ITS sequence analysis of 29 predo minant strains.The fungi were identified into 23 genus and 1 family,23 strains belong to Epulorhiza,Tulasnella,Rhizoctonia,Fusarium,Trametes,Trichaptum, Daedaleopsis, Psathyrella, Fomitopsi, Chaetomium, Pleurotus, Lecanicillium,Eutypella,Lecanicillium,Peniophorella,which their frequency were 16% and 19%.Only one family belonged to Tulasnellaceae.12 strains of dominant mycorrhiza had different microcosmic and macroscopic,none of them had conidiophore,near the hyphal branching-points formed of septum and conjugate nuclei,11 strains mycorrhiza with intestiniform cells,the most of mycorrhiza had a distinct aroma.
【Fund】: 贵州省农业攻关资助项目(黔科合支撑[2017]2524);; 贵州省林业厅青年人才基金资助项目(黔林科合J[2013]2号);; 贵州乡土珍稀花卉研发团队服务企业行动计划资助项目(黔科合平台人才[2016]5711);; 贵州省社会发展资助项目(黔科合SY字[2015]3019号)
【CateGory Index】: S682.31
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