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《Northern Horticulture》 2017-24
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Identification and Determination of Diterpene Lactone in Andrographis paniculata Nees by TLC and HPLC

LIAN Ruili;LI Yuwei;MA Xia;GENG Yuanbang;LIU Yonglu;ZHANG Guozu;Animal Husbandry and Economy of Henan University;Henan Kangxing Pharmaceutical Company Limited;  
With Andrographis paniculata as test material,andrographolide and dehydroandrographolide were identified by TLC,using chloroform-ethyl acetate-methanol(4∶3∶0.4)as the mobile phase and2%3,5-diniotrbenzoie acid ethanol-2 mol·L-1 potassium hydroxide(1∶1)as the chromogenic agent;and the content of andrographolide and dehydroandrographolide was detected by HPLC.The separation was carried out on a Hypersil BDS C18(4.6 mm×250 mm,5μm)column with the mobile phase of methanol-water(65∶35);the column temperature was at 30 ℃;the detection wavelength of andrographolide and dehydroandrographolide were 225 nm and 254 nm,respectively.The results showed that andrographolide and dehydroandrographolide could be identified by TLC,the content of andrographolide and dehydroandrographolide in Andrographis paniculata could be accurately determined by HPLC.The established methods of TLC and HPLC might be a reliable and specific method with good reproducibility,which could be used as quality control method of Andrographis paniculata.
【Fund】: 河南省科技攻关计划资助项目(162102310434);; 河南省中草药组培快繁及资源开发利用科技团队资助项目(C20150033);; 河南牧业经济学院科技创新团队资助项目(HUAHE2015009)
【CateGory Index】: R284.1
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