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《Chinese Journal of Zoology》 2002-06
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Preliminary Study of Rabbit Somatic Cell Nuclear Transfer

HOU Jian\ AN Xiao Rong GOU Ke Mian GUAN Hong\ BAI Jia Lin LI Rui Guo CUI Xiu Hong CHEN Yong Fu (National Key Laboratory for Agrobiotechnologies, China Agricultural University Beijing 100094,China)  
The experiments described in this paper aimed to establish a basic protocol for somatic cell nuclear transfer in the rabbit. By testing the effects of different electric pulses (EP) on fusion of rabbit 2 cell embryo blastomeres and activation of rabbit oocytes, it was found that 200 and 260?V/mm EP were effective. When employed in nuclear transfer using rabbit fetal fibroblast cells as donors, 200 and 260?V/mm EP resulted in 44 4% and 48 4% fusion, 58 8% and 53 8% cleavage, 5 9% and 5 5% morulae/blastocysts, respectively. However, the transfer of 112 reconstructed rabbit embryos to 5 recipient rabbits did not result in a single successful pregnancy. The results indicate that the protocol established in this study can support the early development of cloned embryos from rabbit fetal fibroblast cells.
【Fund】: 国家“8 6 3”计划资助项目 (No .Z2 1 0 1 0 1)
【CateGory Index】: S814.8
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【References】
Chinese Journal Full-text Database 2 Hits
1 WANG Chun-Yu~1, LU Jin~2, WU Hao~2, WEI Xin~1, ZHAO Fu-Tao~1, JIA Zhan-Sheng~1~1Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China, ~2Northwest Sci-Tech University of Agriculture and Forestry, Xi'an 712100, China;Superovulation and parthenogenetic activation in rabbits[J];Journal of The Fourth Military Medical University;2005-21
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Hou Jian; An Xiaorong; Gou Kemian; Liu Jiarixi; Zhou Yun; Chen Yongfu (National Laboratories for Agrobiotechnology, China Agricultural University, Beijing l00094);Rapid Sex Determination of Cultured Rabbit Fetal Fibroblast Cell Lines by Sry-PCR[J];Journal of Agricultural Biotechnology;2001-02
【Co-citations】
Chinese Journal Full-text Database 8 Hits
1 WANG Yi et al(Center of Comparative Medicine,Yangzhou University,Yangzhou,Jiangsu 225009);Determination on Sex of Goat Fibroblast Cell with SRY Gene[J];Journal of Anhui Agricultural Sciences;2009-16
2 LIU Ya,ZHANG Xiao rong,WU Ji yi (Animal Husbandry Aquiculture College of AnHui Agriculture University,230036,China);Progress of Somatic Nuclear Transfer in Mammal[J];Progress In Veterinary Medicine;2002-06
3 CUI Wen-tao1, JIN Er-hui1, LI Kui1, CHEN Yong-fu2 (1.Institute of Animal Sciences, Chinese Academy of Agricultural Sciences,Beijing 100094,China; 2.State Key Laboratory for Agrobiotechnology, China Agricultural University,Beijing 100094,China);Progress in Transgenic Sheep[J];Journal of Agricultural Biotechnology;2007-03
4 GOU Ke Mian 1 AN Xiao Rong 1 TIAN Jian Hui 2 CHEN Yong Fu 1 ( 1National Key Laboratories for Agrobiotechnology, 2College of Animal Science and Technology,China Agricultural University, Beijing 100094, China);Transgenic Animals Bioreactors[J];Chinese Journal of Biotechnology;2002-02
5 Hou Jian An Xiaorong Guan Hong Gou Kemian Lin Aixing Chen Yongfu (National Key Laboratory for Agrobiotechnologies China Agricultural University, Beijing 100094);Cell Cloning from Transfected Rabbit Primary Cell Lines and Analysis of Their Ploidy[J];Biotechnology Information;2002-05
6 AN Xin Min XU Chang Jie ZHANG Shang Long TAO Jun CHEN Jun Wei ( Department of Horticulture, Huajiachi Campus, Zhejiang University, Hangzhou 310029; Department of Horticulture, Yangzhou University, Yangzhou 225009);QUICK SCREENING CITRUS SINENSIS GENOMIC DNA LIBRARY AND CLONING BY FILTER PAPER ABSORPTION-PCR AND IMPROVED SUBCLONING METHOD[J];Chinese Journal of Coll Biology;2003-01
7 Yang YD, Zhang WY, Fang GJ, Wo EK, He ZJ, Hong Y, Chen Y Institute of Bioengineering, Zhejiang Academy of Medical Sciences, Hangzhou 310013, Zhejiang Province, China;Cell sources for cartilage defect repair with rabbit bone marrow mesenchymal stem cells:PCR amplification for sex-determining gene sequence[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2007-37
8 LI Xiang-chen,YUE Hua,LIU Peng,WU Yun Qi Qi Ge,GUAN Wei-jun,MA Yue-hui(Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100094,China);The Research of the Impact of the State of Donor Cell on the Growth of Reorganization Embryos[J];Chinese Journal of Animal and Veterinary Sciences;2008-04
【Co-references】
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1 SHANG Jiang-hua*, XU Ru-xiang*, JIANG Xiao-dan*, LEI An-min, ZOU Yu-xi*, QIN Ling-sha*, TENG Xiao-hua*, ZHENG Xing, CUI Sheng. *Neuromedical Institute, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China;Preliminary research on nuclear transfer by using bone marrow stromal cells as nuclear donors in rabbits[J];Chinese Journal of Neuromedicine;2005-09
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3 YAN Quan Jian 1, LI Liu Jin 2, YANG Gui Zhong 2, ZHANG Yu Mei 2, WANG Yue Feng 2, WANG He 1, SHAO Guo Xing 1 ( 1Department of Urology, Xijing Hospital, 2Laboratory Animal Center, Fourth Military Medical University, Xi'an 710033, China;Production of blastocysts by transfer of nuclei from nonquiescent adult rabbits bone mesenchymal stem cells and adipose stromal stem cells[J];Journal of The Fourth Military Medical University;2002-05
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【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 LIUMu1,2, GaoShan1 , Sun Jing- jiang1, LIUYun- bo1 ( 1.Institute ofLaboratoryAnimal Sciences, Chinese AcademyofMedical Sciences( CAPM) &PekingUnion Medical College ( PUMC) , Beijing, 100021; 2.Chinese Center ofDisease Contro(l CCDC) , Beijing, 100021);Studyon the superovulation ofrabbit in China(2)[J];Heilongjiang Journal of Animal Reproduction;2008-05
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