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《Chinese Journal of Zoology》 2007-01
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Sequence Analysis and Molecular Phylogeny of Mitochondrial 16S rRNA Gene Fragments in Three Populations of Macrobrachium nipponense

SUN Yue-Na① FENG Jian-Bin① LI Jia-Le ①* NIE Shi-Zhong② (① The Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecology Certificated bythe Ministry of Agriculture, Shanghai Fisheries University, Shanghai 200090;② Changxing Fisheries Technology Station, Changxing Zhejiang 313100, China)  
Mitochondrial 16S rRNA gene fragments of wild Macrobrachium nipponense were amplified with PCR,then the PCR products were purified and sequenced,and 495 bp nucleotide sequences were obtained.The A,T,G and C contents in this fragment were 28.6%,36.1%,22.7% and 12.5%,respectively; the AT content was higher than the GC content.Little sequence variation was observed in 16S rRNA gene fragments within species,and transition in only 5 loci was revealed in three populations.Furthermore,454 bp fragment of the sequence was used to discuss the phylogenetic relationship among M.nipponense,M.rosenbergii,M.hainanense,M.malcolmsonii, M.lar,M.latimanus,M.carcinus and M.equidens using Fenneropenaeus chinensis as the out-group.The molecular phylogenetic tree was constructed by NJ method using software MEGA 3.1,and three populations of M.nipponense were clustered first and then clustered with M.hainanense; M.rosenbergii and M.malcolmsonii,as well as M.lar and M.latimanus had a closer genetic relationship and were clustered first,then they were clustered with M.carcinus and M.equidens,and finally with Fenneropenaeus chinensis.
【Fund】: 浙江省科技攻关计划项目(No.2004C32054);; 上海市水产办项目(No.2005-06);; 上海市重点学科建设项目资助(No.Y1101)
【CateGory Index】: Q953;Q951;S917.4
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3 LU Jian\|ping, JIANG Nai\|cheng (College of Life Science, Zhejiang University,Hangzhou 310012, China);The isozyme patterns in embryo development of the freshwater prawn Macrobrachium rosenbergh[J];DONGHAI MARINE SCIENCE;2000-03
4 WANG Jin ke 1 , ZHOU Gang 2, CAO Wen ming 2, HU Ben long 3, XING Yi yu 3 (1. Institute of Fisheries Science, College of Life Science, Nanjing Normal University, Nanjing 210097, China; 2. Institute of Freshwater Fishery of Jiangshu Province;Genetic polymorphism of Chinese mitten-handed crabs (Eriocheir sinensis) with DNA fingerprinting produced by minisatellite probe 33.6[J];Journal of Dalian Fisheries University;2001-02
5 <Title>Qiu tao; Lu renhou; Xiang chaomei and Zhang jin(Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan 420072);STUDIES ON THE GENOMIC DNA GENETIC DIVERSITY IN THREE POPULATIONS OF Eriocheir sinensis USING RAPD[J];FRESHWATER FISHERIES;1997-05
6 SONG She-Wu ZHAO Tong\|Yan\ DONG Yan\|De\ JIANG Shu\|Nan *\ LU Bao\|Lin( Institute of Microbiology & Epidemiology, AMMS, Beijing 100071) *Department of Plant Protection,Southwest Agricultural University,Beibei,Chongqing 400716.;mtDNA-16S rRNA SEQUENCES OF THE CULEX PIPIENS COMPLEX AND THEIR PHYLOGENETIC ANALYSES[J];Acta Zootaxonomica Sinica;2002-04
7 QIU Gao feng CHANG Lin rui XU Qiao ting FANG Xiong ying LOU Yun dong (Fisheries College,Shanghai Fisheries University,Shanghai 200090,China qiugf@online sh cn);INTRASPECIFIC DNA SEQUENCE POLYMORPHISM IN THE MITOCHONDRIAL 16SrRNA GENE OF THE CHINESE SHRIMP,Penaeus chinensis[J];ZOOLOGICAL RESEARCH;2000-01
8 GU Xiaohong 1 ZHAO Fushun 2 (1:Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P.R.China; 2:Nanjing Vegetable-Seed Company, Nanjing 210042, P.R.China);Resources and Culturing Situation of Chinese Mitten Crab (Eriocheir sinensis) and Species Character Conservation[J];Journal of Lake Sciences;2001-03
9 Sun Zhenxing~(1)) Song Zhile~(2)) Li Nuo~(1)) Zhao Yushan~(2)) Guan Xianghua~(2)) (1) Shandong School of Fisheries (2) Zhifu Fisheries Bureau, Yantai);A PRELIMINARY STUDY ON THE GROWTH OF TRIPLOID ABALONE(Haliotis discus hannai Ino)[J];Transactions of Oceanology and Limnology;1992-04
10 GAO Tian-xiang ;ZHANG Xiu-mei(Fisheries College, Ocean University of Qingdao, Qingdao, 266003);GENETIC VARIATION OF STOCKED POPULATION OF JAPANESE MITTEN CRAB ERIOCHEIR JAPONICA[J];TRANSACTION OF OCEANOLOGY AND LIMNOLOGY;1999-03
China Proceedings of conference Full-text Database 1 Hits
1 KONG Xiaoyu, LIU Yajun, YU Ziniu, SONG Linsheng(1. The Key Laboratory of Mariculture, Ministry of Education, Ocean Unviersity of Qingdao, Qingdao 266003, China 2. Institute of Oceanology, Academy of sciences, Qingdao 266071);Comparative study of mitochondrial 16S rRNA gene fragments of Scallop Chlamys farreri and Argopecten irradians[A];CHINESE SOCIETY FOR OCEANOLOGY AND LIMNOLOGY (缩写CSOL)[C];1999
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