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《Chinese Journal of Zoology》 2007-01
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Primary Culture of Male Germ Cells in Macrobrachium nipponense

GUO Zhong-Hai KANG Xian-Jiang* MU Shu-Mei SUN Jing GUO Ming-Shen (College of Life Science,Hebei University,Baoding 071002,China)  
Cell culture is a significant tool for studying endocrinology and diseases in shrimp.A primary male germ cell culture method in Macrobrachium nipponense is introduced in this paper.We found that spermatogonia and spermatocytes had active division capability.According to the data about cell adherence and cell growth,we determined the optimal pH and osmolarity for in vitro culture of these cells.The optimal pH was 7.2-7.4 and the proper osmolarity was 400-500 mmol/L.
【Fund】: 国家自然科学基金资助项目(No.30371115)
【CateGory Index】: Q813.11
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