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《Chinese Journal of Zoology》 2011-03
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Isolation of Two Growth Hormone Receptor Genes and SNPs Associated with Body Weight in GIFT Strain Tilapia Oreochromis niloticus

RUAN Rui-Xia① YU Ju-Hua② LI Hong-Xia② LI Jian-Lin② TANG Yong-Kai② WEI Kei-Peng① ① Wuxi Fishery College,Nanjing Agricultural University,Wuxi 214081; ② Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes,Ministry of Agriculture, Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China  
Growth hormone receptors(GHR) which located on the cell membrane is the only pathway for growth hormone(GH) to get into cells and affect the growth in animals,they play a vital important role on animal growth.Two cDNA sequences and DNA sequences of exon 2-9 of GHR1,exon 2-8 of GHR2 were isolated from GIFT strain(Genetically Improved Farmed Tilapia strain,Oreochromis niloticus) using RT-PCR and PCR in this study.Six and sixteen SNPs were identified on GHR1 and GHR2 respectively,only two of which were located at exons.Genotypes were test in eight SNPs(GHR1 intron 3_A612G,intron 3_A989T,intron 7_A599C;GHR2 intron 3_C330T,intron 3_A645G,intron 3_G687T,intron 3_A967G,intron 7_C107T) of 120 individuals from five families of GIFT strain by PCR-RFLP.Correlation analysis between genotypes and weight gain showed that GHR1 intron 3_A612G and intron 3_A989T associated with male fish weight gain(P0.01 and P0.05) significantly.Similar situation was found in the female population,but the difference was not significant(P0.05).Intron 7_A599C was significantly associated with both male(P0.01) and female(P0.05) fish weight gain.The CC type fish weight gain was significantly higher than AA type's.On the GHR2,only intron 3_G687T was found to correlate with female weight gain significantly(P0.05).The GT type female fish gain was significantly higher than GG type's.The correlation between genotypes of 3_A612G,3_A989T,7_A599C and 3_G687T and weight gain was detected in 356 individuals from 60 families of GIFT strain(6 individuals in average of each family).There were similar growth situation among different genotypes' fish,the decreased significance may result in the diverse genetic background and smaller number of samples.Real-time quantitative PCR results indicated that GHR1 expression in liver of male fish was 1.36 times higher than that in female's liver,the male fish GHR2 expression in the tissues(except kidney) was lower than that in the female,which can explain the different effects of GHR1 and GHR2 SNP loci on the weight gain of male and female GIFT.In addition,the different influences of GHR1 and GHR2 on the growth result in that only one SNP loci on GHR2 could affect the weight gain.
【Fund】: 中央级公益性科研院所基本科研业务费专项资金(No.2011JBFC03 2009JBFB05);; 农业部淡水生物多样性保护与利用重点开放实验室第七期开放课题(No.LFBU0706);; 公益性行业(农业)科研专项经费项目(No.200903046-02)
【CateGory Index】: S917.4
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【Citations】
Chinese Journal Full-text Database 5 Hits
1 DONG Zai-jie1,2,HE Jie1,ZHU Jian1,ZHANG Shou-ling1,ZHANG Cheng-feng1,XU Pao1,2,NONG Xiao-ying2,LIANG Zheng-yuan2,MIAO Ling-hong2(1.Key Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081;2.Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081);Comparison of Growth among 60 Families GIFT Strain Tilapia at Initial Stage[J];Freshwater Fisheries;2008-03
2 NI Jing1,2,YOU Feng1,YU Shen-Hui1,2,ZHANG Pei-Jun1,XU Dong-Dong1,2,WU Zhi-Hao1,2,WEN Ai-Yun,XU Yong-Li(1.Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;2.Graduate University of Chinese Academy of Sciences,Beijing 100039,China);Microsatellite Polymorphism Analysis of GHR Gene Promoter Region in Paralichthys olivaceus Hatchery Stocks and Its Association with Growth Traits[J];Periodical of Ocean University of China;2008-05
3 YU Ju-Hua,LI Hong-Xia,TANG Yong-Kai,LI Jian-Lin and DONG Zai-Jie(Key Laboratory for Genetic Breeding and Aquaculture Biology of Freshwater Fishes,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China);ISOLATION,TRANSCRIPTS POLYMORPHISM AND TISSUES EXPRESSION OF GROWTH HORMONE RECEPTOR GENES IN CYPRINUS CARPIO VAR. JIAN[J];Acta Hydrobiologica Sinica;2011-02
4 ZHAO Gao-Feng1,CHEN Hong1,2,LEI Chu-Zhao1,ZHANG Chun-Lei1,ZHANG Li1,LAN Xian-Yong1,FANG Xing-Tang2,KANG Xiang-Tao1 1.College of Animal Science and Technology,Northwest A&F University,Shaanxi Key Laboratory of Molecular Biology for Agriculture,Yangling,712100,China;2.Institute of Cellular and Molecular Biology,Xuzhou Normal University,Xuzhou 221116,China;Study on SNPs of GHR gene and its associations with growth traits in Qinchuan cattle[J];Hereditas;2007-03
5 LU Yong-quan1,2, WANG Xu-sheng1, HUANG Wei-su1, XIAO Tian-xia1, ZHENG Yan1, WU Wei-ren1,3 (1College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029; 2Biotechnology Research Center, Heilongjiang Academy of Agricultural Sciences, Haerbin 150086; 3College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002);Development of Amplified Consensus Genetic Markers in Gramineae Based on Rice Intron Length Polymorphisms[J];Scientia Agricultura Sinica;2006-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Xing-wei et al (Tobacco Research Institute, CAAS, Qingdao, Shandong 266101);IP Marker and Its Application Strategy in Molecular Breeding of Tobacco[J];Journal of Anhui Agricultural Sciences;2008-08
2 ZHANG Chun-zhen et al(Ningxia University,Yinchuan,Ningxia 750021);Analysis on Polymorphisms of GHR Gene in Improved Hybrid Yellow Cattle Groups from Liupan Mountain Area in Ningxia[J];Journal of Anhui Agricultural Sciences;2011-13
3 ZHANG Li-xin CHEN Hong-quan WANG Li-sheng*(Animal Science and Technology College of Anhui Agricultural University,Hefei Anhui 230036);Research Advances of Polymorphism of Growth Hormone Receptor Gene in Cattle[J];;2009-11
4 CHEN Xue-Feng①② YANG Guo-Liang② YU Ju-Hua③ TANG Yong-Kai③ LI Jian-Lin③ LI Hong-Xia③ (① Wuxi Fishery College,Nanjing Agricultural University,Wuxi 214081; ② Zhejiang Institute of Freshwater Fishers and Zhejiang South Tai-lake Freshwater Fish Breeding Co. ,Ltd,;Huzhou 313001; ③ Key Laboratory of Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agriculture, Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China);Isolation of IGF2 Gene and Correlation of Its SNPs with Fish Sharp and Weight Gain in GIFT Strain Nile Tilapia Oreochromis niloticus[J];Chinese Journal of Zoology;2010-02
5 DONG Zai-Jie①②③ LIANG Zheng-Yuan③ LIU Jie-Qi③ SU Sheng-Yan②MING Jun-Chao③ QU Jiang-Qi③ XU Pao②③ XIE Zhuang①(① College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095;② Key Open Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes,Ministry of Agriculture;Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081;③ Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081,China);Genetic Diversity Analysis in A Newly Introduced Population of GIFT Strain Nile Tilapia (Oreochromis niloticus)[J];Chinese Journal of Zoology;2010-05
6 CHEN Hong1,2,ZHANG Chun-lei2(1.College of Animal Science and Technology,Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology,Yangling,Shaanxi 712100,China;2.Institute of Cellular and Molecular Biology,Xuzhou Normal University,3.Xuzhou,Jiangsu 221116,China);Recent Progress in Beef Cattle Molecular Breeding in China[J];china Cattle Science;2008-04
7 MA Yun1,MA Wei2,LI Ai-min2,LI Rong-rong2,CHEN Hong2(1.College of Life Science,Xinyang Normal University,Xinyang,Henan 464000;2.College of Animal Science and Technology,Northwest A & F University,Yangling 712100,China);Progress on Molecular Markers in Qinchuan Cattle[J];China Cattle Science;2011-02
8 HE Xin1,4,LIU Guo-dao2,LIU Di-qiu3,LUO Fu-cheng4,HUANG Bi-zhi1(1.Yunnan Beef Cattle & Pasture Research Centre,Kunming 650212,China;2.Tropical Pasture Research Center,Tropical Crops Genetic Resources Institute,CATAS,Danzhou 571737,China;3.College of Life Science & Technology,Kunming University of Science and Technology,Kunming 650224,China;4.College Animal Science & Technology,Yunnan Agricultural University,Kunming 650201,China);Analysis of the genetic diversity of Desmodium genus using transferred ACGM and EST-SSR markers[J];Acta Prataculturae Sinica;2008-06
9 Dong Dezhen ( Zhejiang Research Institute of Chemical Industry, Hangzhou 310023, P. R. China); Wu Licheng, Xia Shanyong, Wang Honglei, Lu Yongquan(Heilongjiang Academy of Agricultural Sciences);Transferability of ACGM in Bamboo[J];Journal of Northeast Forestry University;2007-01
10 LI Ju-cai1,ZHANG Chun-zhen2,YANG Yi2,YU Jian-yong3(1.Research Center of Grass and Livestock of Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002,China;2.Ningxia University,Yinchuan 750021,China;3.Office of Agro-farming and Science Technology of Jingyuan County,Jingyuan 756400,China);Study on Polymorphisms of GHR Gene in Improved Hybrid Beef Cattle Groups in Ningxia Province[J];China Animal Husbandry & Veterinary Medicine;2011-07
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Yong\|Qing ① WANG Xin\|Guo ② XU Lai\|Xiang ① ZHANG Zhi\|Bin ① (①Institute of Zoology,Chinese Academy of Sciences\ Beijing\ 100080; ②Institute of Biological Engineering\ Beijing\ 100071, China);A New Rapid Method for Extraction of High Quality of Genomic DNA from Animal Tissues[J];Chinese Journal of Zoology;2001-01
2 CHEN Hong, ZHANG Yinghan (College of Animal Science and Veterinary Medicine,Northwest Sci Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China);Selection and Breeding for Beef Performance in Qinchuan Cattle and Technical Measures[J];Journal of Yellow Cattle Science;2002-02
3 CONG Lin-lin1,2,SONG Guang-jun2,MU Yun-lei2,CHEN Shu-jun1,GE Long-li3,GAO Xiang-gang2,HE Chong-bo2(1.College of Life Science,Liaoning Normal University,Dalian 116029,China;2.Liaoning Ocean and Fisheries Science Research Institute,Liaoning Open Lab of Applied Marine Biotechnology,Liaoning Key Lab of Marine Fishery Molecular Biology,Dalian 116023,China;3.College of Life Science and Technology,Dalian Fisheries University,Dalian 116023,China);The 12S and 16S rRNA Sequences and Phylogenetic Analysis of Barfin Flounder Verasper moseri and Spotted Halibut V.variegatus[J];Fisheries Science;2007-07
4 MA Hongyu , YUE Yongsheng , GUO Jinfeng , GONG Weihua & WANG Hui ( Molecular genetics and animal breeding Lab, Shandong Agricultural University, Tai'an 271018, P. R. China) (Institute of Animal Science ,Chinese Academy of Agricultural Sciences, Beijing 100094, P. R. China );Microsatellite marker analysis of genetic diversity and phylogenetic relationships in three populations of Cypnnus carpio L. , Shandong Province[J];Journal of Lake Sciences;2006-06
5 YOU Feng,\ WANG Ke-Ling, XIANG Jian-Hai, XU Cheng (Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071);COMPARATIVE ANALYSIS OF BIOCHEMICAL GENETIC STRUCTURE AND VAR IANCE BETWEEN NATURAL AND CULTURED STOCKS ON THE LEFT-EYED FLOUNDER, PARALICHTHYS OLIVACEUS (T.& S.) OFF SHANDONG COASTAL WATERS[J];Oceanologia Et Limnologia Sinica;2001-05
6 WANG Wei, YOU Feng , GAO Tian-Xiang , ZHANG Pei-Jun  (Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071; College of Life Sciences and Technology, Ocean University of China, Qingdao, 266003) (Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071) (College of Life Sciences and Technology, Ocean University of China, Qingdao, 266003);GENETIC VARIATIONS AT TEN MICROSATELLITE LOCI IN NATURAL AND CULTURED STOCKS OF LEFT-EYED FLOUNDER PARALICHTHYS OLIVACEUS IN SHANDONG COASTAL WATERS[J];Oceanologia Et Limnologia Sinica;2004-06
7 LI Qi(The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China);Advances in Studies on Microsatellite Markers in the Pacific Abalone[J];Journal of Ocean University of Qingdao;2004-03
8 SHU Jing1,2,LI Qi1,YU Rui-Hai1,TIAN Chuan-Yuan1(1.The Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,China;2.The Key Laboratory for Sustainable Vtilization of Marine Fisheries Resources,Ministry of Agriculture, Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China);Microsatellites Analysis on Genetic Variation Between Wild and Cultured Populations of Pacific Abalone(Haliotis discus hannai)[J];Periodical of Ocean University of China;2008-01
9 LI Sifa LI Jiale LI Chenghong ( Key Laboratory of Ecology and Physiology in Aquaculture, Ministry of Agriculture, Shanghai Fisheries University, 200090 )HAN Fengjin ( National Tilapia Seed Farm of Qingdao, Qingdao 266317 )YE Wei ( National Til;On-Farm Evaluation of GIFT Strain of Nile Tilapia[J];FISHERIES SCIENCE & TECHNOLOGY INFORMATION;1997-06
10 YU Ju-hua, XIA De-quan, YANG Hong, HE Yan-hui, CHEN Yong-jun, WU Ting-ting (Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences , Wuxi 214081, China ; College of Fisheries , Nanjing Agriculture University, Wuxi 214081, China);Isolation and sequence somatostatin cDNA of Megalobrama pellegrini[J];Journal of Fisheries of China;2003-06
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