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《Chinese Journal of Zoology》 2011-03
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Genetic Diversity Analysis of D-loop Region in Populations of Paramesotriton caudopunclatus

CHEN Guang-Zhao XIAO Yu CHEN Xue-Ping GU Xiao-Ming School of Life Sciences,Guizhou Normal University,Guiyang 550001,China  
We studied the genetic diversity of five populations of Paramesotriton caudopunclatus using D-loop sequence of mtDNA as a molecular marker.As a result,we obtained 690 bp long D-loop sequences of 42 samples from five populations collected from Leigongshan and Wulingshan.In the sequences,there were 30 variable sites.Based on these sites 13 haplotypes are defined.No shared haplotype was found among the five populations.P.caudopunclatus populations had high haplotype diversity and low nucleotide diversity.The results of fixtion index(FST),nucleotide diversity(Dxy) and migration rates(Nm) analyses indicated that a certain extent differentiation occurred between Leigongshan population and Wulingshan population.In phylogenetic trees,all haplotypes were clustered according to their geographic distribution.Three populations from Leigongshan constituted one clade,while the other two populations from Wulingshan constituted another clade,supporting the treatment of Leigongshan and Wulingshan populations as two separate management units.
【Fund】: 贵州省教育厅自然科学研究项目(No.黔教科(2007)017);; 贵州省国际科技合作重点项目(No.黔科合外G字(2008)700116)
【CateGory Index】: Q953
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