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《Chinese Journal of Zoology》 2011-06
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The Fluorescent Staining of Mollusks Haemocytes with Thioflavin T

WANG Yi-Yan SONG Xiao-Na FU Jing-Yun SUN Hu-Shan College of Life Sciences,Ludong University,Yantai 264025,China  
Thioflavin(TFT),a benzothiazole fluorescent dye,is able to selectively stain amyloid structures and is mainly used to detect amyloidosis using fluorescence microscopy.In this study,the circulating hemocytes of four species of mollusks,Chlamys farreri(Bivalvia),Mactra chinensis(Bivalvia),Natica janthostomoides(Gastropoda) and Octopus variabilis(Cephalopoda),were observed in order to develop the fluorescence staining method of mollusks haemocytes with TFT.Under fluorescence microscope,haemocytes of four mollusks showed yellow-green fluorescence after treatment with 0.1% TFT.The outlines of the cells were clear;the nuclei and granules in the cytoplasm were clearly distinguishable.The haemocytes of all four mollusks could be divided into two types,granulocytes and agranulocytes,based on whether cytoplasmic granules could be observed.In scallop,clam and octopus,the agranulocytes could be subdivided into hyalinocytes and haemoblasts,while the granulocytes could be subdivided into small granulocytes and large granulocytes according to the size of granules in the cytoplasm.The fluorescence staining method of mollusks haemocytes with TFT is easy to operate,and the samples can be observed for a long time after staining because the fluorescence is not easy to bleach.This study is the first to use TFT in the morphological characterization and classification of mollusks haemocytes,and suggests that TFT fluorescence staining is a good method for the observation and classification of mollusks haemocytes.
【Fund】: 山东省自然科学基金项目(No.ZR2010CM017)
【CateGory Index】: Q952
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【Citations】
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【Co-citations】
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1 NING Jun-hao;QIN Yu-bo;HU Lun-chao;ZHANG Wei-jie;LI Lie;CHANG Ya-qing;SONG Jian;Key Laboratory of Mariculture & Stock Enhancement in North China's Sea,Ministry of Agriculture,Dalian Ocean University;Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences;Liaoning Province Marine Environmental Forecasting Center;;Effects of abrupt and gradual decreases in water temperature on blood physiological and biochemical parameters in dusty rabbit fish Siganus fuscescens[J];大连海洋大学学报;2017-03
2 ZHANG Zhenyu;WANG Qiurong;YE Kun;WANG Zhiyong;Key Laboratory of Healthy Mariculture for the East China Sea,Ministry of Agriculture,Fisheries College,Jimei University;;Effect of fasting on body composition,muscle fatty acid profiles and serum biochemical parameters of large yellow croaker(Larimichthys crocea)[J];水产学报;2016-09
3 LIU Qunfang;CAO Junming;HUANG Yanhua;WANG Guoxia;MO Wenyan;ZHOU Tingting;SUN Zhiwu;LIU Xiaoling;College of Animal Science, South China Agricultural University;Institute of Animal Science, Guangdong Academy of Agricultural Sciences;Guangdong Public Laboratory of Animal Breeding and Nutrition;Guangdong Key Laboratory of Animal Breeding and Nutrition;;The combined effects of β-glucan with selenium and vitamin E on the growth performance, serum immune and antioxidant indexes, and disease resistance of Litopenaeus vannamei[J];中国水产科学;2013-05
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5 SHI Zhao-hong1,2*,PENG Shi-ming1,SONG Guo1,2,SUN Peng1,YIN Fei1,WANG Jian-gang1(1.Key and Open Laboratory of Marine and Estuary Fisheries,East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Shanghai 200090,China; 2.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China);The effects of starvation and refeeding on tissue and serum metabolic enzyme activities and glycogen contents of barred knifejaw(Oplegnathus fasciatus)[J];水产学报;2012-09
6 CHEN Chao1,SHI Zhao-hong2,XUE Bao-gui1,3,WANG Lu1,LI Yan-lu1,3,WU Lei-ming1,3,QU Jiang-bo4,Ma Ai-jun1,(1.Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;2.East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China;3.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;4.Yantai Tianyuan Aquatic Limited Corporation,Yantai 264003,China);Influence of low-temperature stress on serum biochemical parameters in juvenile Epinephelus septemfasciatus[J];水产学报;2012-08
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10 Bai Xiu-juan et al;Influence of Nitrite to the Growth,Phosphatase and Phenoloxidase of Branchiostoma belcheri Gray[J];齐鲁渔业;2009-08
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