Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Zoology》 2019-03
Add to Favorite Get Latest Update

Isolation and Identification of Primary Microglia from Tree Shrew

LI Xiao-Hui;LI Ming-Xue;HUANG Xing;ZHANG Zhi-Cheng;YUAN Yuan;DAI Jie-Jie;Chinese Academy of Medical Sciences/Peking Union Medical College, Institute of Medical Biology;Chinese Academy of Medical Sciences Institute of Medical Biology, Kunming Medical University;  
The aim of this study is to establish a method for purifying and culturing cerebral microglial cells of tree shrew(Tupaia belangeri), providing new experimental materials for future research. Newborn tree shrews were used in this experiment. The cortex of cerebrum was isolated and disaggregated by trypsin digestion. The microglia cells were purified by three methods after culturing for 9-10 days: upright hand clapping, mild digestion with trypsin and constant temperature oscillation. Meanwhile,we also used differential attachment method to further purify cells. At last, the number of purified cells was counted using a cell counter. Purified microglial cells were labeled with specific marker CD11b and observed with fluorescence microscope. The results showed that microglial cells were in a resting state on the third day of isolation and culture, with irregular shapes such as spindle, rod and branch(Fig. 2). Purified microglial cells were positively labeled with specific marker CD11b as revealed by fluorescence microscopy(Fig. 3). Data statistics was performed using SPSS statistical software. Analysis of cellular immunofluorescence intensities and counting of cells purified by different purification methods showed that the cell yield obtained by the upright hand clapping method was significantly higher than that obtained by constant temperature oscillation method, and the positive cell rate was significantly higher than that obtained by mild trypsin digestion method(Fig. 4). Primary microglial cells of tree shrews with high yield and purity were obtained by upright hand clapping.
【Fund】: 云南省科技人才和平台计划项目(No.2017HC019);; 云南省重大科技专项(No.2017ZF007);; 国家科技支撑计划项目(No.2014BAI01B00);; 云南省联合支持国家计划项目(No.2015GA009)
【CateGory Index】: Q813.11
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 8 Hits
1 Zhang Liqing;Zhang Zhangang;Xu Ying;Department of Physiology,Shanghai University of Traditional Chinese Medicine;;A improved method for cultivating primary microglia of the rat[J];神经解剖学杂志;2015-03
2 CHEN Xue;ZHANG Ting;LI Cai-hong;WANG Wei;LUO Xiang;YU Zhi-yuan;Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology;;Primary Culture and Purification of Microglia from Rat Spinal Cord[J];神经损伤与功能重建;2014-06
3 SU Ao-lei;QIN Yin-ge;ZHENG Yu;CHEN Qun;YUN Chen-xia;HUANG Zheng-tuan;LENG Jing;Guangxi University of Chinese Medicine Graduate School;Guangxi University of Chinese Medicine;;Introduction to Biological Characteristics of Tree Shrew[J];动物医学进展;2014-10
4 Kong Huimin,Gan Na,Peng Jing,He Fang,Ma Yuping,Yin Fei(Department of Pediatrics,Xiangya Hospital,Central South University,Changsha 410008,China);A modified method for neonatal rat microglia primary culture[J];神经解剖学杂志;2013-04
5 HUANG Xiao-yan,XU Juan,SUN Xiao-mei,DAI Jie-jie(Institute of Medical Biology,the Chinese Academy of Medical Science and Peking Union Medical College.Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases.Kunming 650118,China);Development of Application of Tree Shrew in Human Disease Animal Models Research[J];实验动物科学;2013-02
6 Lin XU 1,2,4 , Yun ZHANG 1 , Bin LIANG 1 , Long-Bao Lü 2,3 , Ce-Shi CHEN 1 , Yong-Bin CHEN 1 , Ju-Min ZHOU 1 , Yong-Gang YAO 1,2,4,* 1. Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China; 2. Kunming Primate Research Center, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China; 3. Experimental Animal Core Facility & Kunming Primate Research Center, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China; 4. Tree Shrew Inbreeding Center, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China;Tree shrews under the spot light: emerging model of human diseases[J];动物学研究;2013-02
7 WANG Bao-ping,LI Dong-feng,XU Shu-wen.Graduate School of Southern Medical University,Guangzhou 510515,China Corresponding author: XU Shu-wen.Tel: 0086-020-83827812-70712.;Comparison of different purified methods in culture of primary microglia and neuron culture[J];广东医学;2012-06
8 YU Teng-bo1,CHENG Yong-shuai,KOU De-wei,CHU Yan-chen,WANG Ai-min(1The Affiliated Hospital of Medical College,Qingdao University,Qingdao 266003,P.R.China);Correlation and analysis of microglia primary culture methods[J];山东医药;2008-17
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 MIAO Yu-Run;SONG Qing-Kai;KUANG De-xuan;DAI Jie-jie;Center of Tree Shrew Germplasm Resources,Institute of Medical Biology,the Chinese Academy of Medical Science and Peking Union Medical College,Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases;;Isolation,primary culture and purification of neonatal tree shrew cardiac fibroblasts and cardiac myocytes[J];中国心血管病研究;2017-10
2 WANG Dong-bao;HU Yun-zhang;HU Ning-zhu;HAN Yuan-yuan;KUANG De-xuan;LI Yan-han;Institute of Medical Biology,Chinese Academy of Medical College and Peking Union Medical College,Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases,Yunnan Research Center of Engineering Technique for Vaccines against Severe Infectious Diseases,Yunnan Key Laboratory of Prevention & Control Research on Insect-Borne Infectious Diseases;;The Immunity Effect Evluation on Tree Shrew by Hepatitis A Virus Attenuated Live Vaccine,Hepatitis B Vaccine Independently or Combined Adjuvant Heparan Sulfate[J];实验动物与比较医学;2017-02
3 MIAO Yu-run;SONG Qing-kai;KUANG De-xuan;CHEN Ling-xia;YIN Bo-wen;LI Xiao-fei;DAI Jie-jie;Center of Tree Shrew Germplasm Resources,Institute of Medical Biology,the Chinese Academy of Medical Science and Peking Union Medical College,Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases;;Primary Isolation,Culture,Purification and Identification of Corneal Epithelial Cells in Tree Shrew[J];实验动物与比较医学;2017-02
4 ZHOU Guang-long;ZHU Qin;LI Zhen-yu;CHEN Ling-xia;YIN Bo-wen;HU Min;SUN Xiao-mei;Department of Ophthalmology,the Second People's Hospital of Yunnan Province & the Fourth Affiliated Hospital of Kunming Medical University;Institute of Medical Biology,Chinese Academy of Medical Sciences & Peking Union Medical College,Tree shrew Germplasm Resource Center;;Progress in the basic ophthalmological research of tree shrew[J];中国实验动物学报;2015-06
5 YIN Bo-wen;CHEN Ling-xia;LI Zhen-yu;LI Na;SUN Xiao-mei;Center of Tree shrews Germplasm Resources,Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Yunnan Key Laboratory of Vaccine Research &Development on Severe Infection Diseases;;The survey of dermatophyte in artificial feeding of tree shrews[J];中国比较医学杂志;2015-11
6 CHEN Ling-xia;YIN Bo-wen;LI Zhen-yu;SUN Xiao-mei;DAI Jie-jie;Chinese Academy of Medical Sciences / Peking Union Medical College,Institute of Medical Biology,Center of Tree shrews Germplasm Resource,Yunnan Key Laboratory of major infectious diseases vaccine development;;Morphological observation and analysis of its 18S rRNA gene of flagellate in tree shrews[J];中国比较医学杂志;2015-11
7 XING Jin;FENG Yu-fang;YUE Bing-fei;HE Zheng-ming;SUN Xiao-mei;DAI Jie-jie;National Institute of Food and Drug Control,Institute of Laboratory Animal Resources;Institute of Medical Biology,Chinese Academy of Medicine Sciences and Peking Union Medical College;;Establishment and application of CODEHOP PCR assay for detection of Haemophilus spp.in tree shrews[J];中国人兽共患病学报;2015-11
8 LIU Li-jun;YU Bing-ting;HU Ning-zhu;SUN Xiao-mei;WANG Wei;SUN Jing;HU Yun-zhang;LI Jian-fang;Kunming Medical University;Institute of Medical Biology,Chinese Academy of Medical Science and Peking Union Medical College;Hebei University;;Isolation,culture and identification of bacterial strains from tree shrews feces[J];中国比较医学杂志;2015-10
9 FANG He;JIN Xiong;WANG Dongdong;Calmette Hospital Affiliated to Kunming Medical University;;Research progress in culture and in vivo and in vitro HBV infection of primary hepatocytes of tree shrews[J];临床肝胆病杂志;2015-10
10 LIN Na;CHEN Mei-Rong;WANG Ting-Hua;Institute of Neuroscience, Kunming Medical University;;Advances of tree shrews in the nervous system diseases[J];生命科学;2015-09
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 HE Bao-li;YANG Ren-hua;LI Jin-tao;LI Bo;WANG Li-mei;JIAO Jian-lin;Department of Laboratory Animal,Kunming Medical University;College of Veterinary Medicine,Yangzhou University;;Pathologial observation of Spontaneous Breast Cancer in Tree Shrews (Tupaia belangeri chinensis)[J];动物医学进展;2013-11
2 XU Ying*,ZHANG Li-qing,XIA Chen-yi,DONG Xian-wen,ZHAO Yan,WANG Xing-yu(Department of Physiology,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China);Effects of Guizhi Plus Gegen Decoction on Spacial Memory and Cytokines and Microglia in Hippocampus of Neuroinflammatory Mice[J];中国实验方剂学杂志;2013-14
3 Ling XU 1,3 , Yu FAN 1,3 , Xue-Long JIANG 2 , Yong-Gang YAO 1, 4,5 ,* 1. Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China; 2. State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China 3. University of the Chinese Academy of Sciences, Beijing 100049, China; 4. Kunming Primate Research Center, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China; 5. Tree Shrew Inbreeding Center, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China;Molecular evidence on the phylogenetic position of tree shrews[J];动物学研究;2013-02
4 Bo LI 1,2 , Rong-Ping ZHANG 1 , Jin-Tao LI 1,2 , Bao-Li HE 1,2 , Hong ZHEN 1,2 , Li-Mei WANG 1 , Jian-Lin JIAO 1,* 1. Laboratory Animal Center of Kunming Medical University, Kunming 650031, China; 2. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;Measurement and analysis of anatomical parameter values in tree shrews[J];动物学研究;2013-02
5 Lin XU 1,2,4 , Yun ZHANG 1 , Bin LIANG 1 , Long-Bao Lü 2,3 , Ce-Shi CHEN 1 , Yong-Bin CHEN 1 , Ju-Min ZHOU 1 , Yong-Gang YAO 1,2,4,* 1. Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China; 2. Kunming Primate Research Center, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China; 3. Experimental Animal Core Facility & Kunming Primate Research Center, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China; 4. Tree Shrew Inbreeding Center, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China;Tree shrews under the spot light: emerging model of human diseases[J];动物学研究;2013-02
6 LI Jin-tao1,2),HE Bao-li1,2),LI Bo1,2),JIAO Jian-lin1),LIU Meng-si3),ZHENG Hong1,2)(1) Dept.of Laboratory Animal Science,Kunming Medical University,Kunming Yunnan 650500;2) School of Veterinary Medicine,Yangzhou University,Yangzhou Jiangsu 225009;3) School of Clinical Medicine,Kunming Medical University,Kunming Yunnan 650031,China);Measurement and Analysis of the Hematology Related Physiological Indicators in Tupaia Belangeri Chinensis[J];昆明医科大学学报;2013-02
7 LIAN Xiu-zhen,ZHANG Hong-mei,XIE Ming-ren,ZHANG Lu,ZHANG Chen,YU Fa-rong* Key Laboratory of Evidence of Science and Technology Research and Application,Gansu Province(Gansu Institute of Political Science and Law),lanzhou 730070,China;Effects of chronic environmental stimulation on behaviors of Chinese tree shrews[J];生态科学;2012-06
8 XING Jin1,FENG Yu-fang1,FU Rui1,YUE Bing-fei1,SUN Xiao-mei1,DAI Jie-jie2,HE Zheng-ming1(1.National Institute of Food and Drug Control,Institute of Laboratory Animal Resources,Beijing 100050,China)(2.Institute of Medical Biology,Chinese Academy of Medicine Sciences(CAMS) & Peking Union Medical College(PUMC),Kunming 650118,China);The Survey of Culturable Bacteria and Fungi in Wild Tree Shrews[J];实验动物科学;2012-03
9 SUN Shan-shan, WANG Bei-bei, JU Li-li, ZENG Hui, XU Qun-yuan. Beijing Institute for Neumscience, Capital Medical University,Beijing Center of Neural Regeneration and Repairing, Key Laboratory for Neurodegenerative Diseases of the Ministry of Education, Beijing 100069, China;Purification of microglia by two-step magnetic activated cell sorting[J];中华实验和临床感染病杂志(电子版);2012-02
10 WANG Bao-ping,LI Dong-feng,XU Shu-wen.Graduate School of Southern Medical University,Guangzhou 510515,China Corresponding author: XU Shu-wen.Tel: 0086-020-83827812-70712.;Comparison of different purified methods in culture of primary microglia and neuron culture[J];广东医学;2012-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved