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Analysis of the Mouse Nodal Gene Promoter on Its 5' Flanking Sequence

HA Xinli\ ZHOU Xunlei (College of Life Sciences.Department of Cell Biology and Genetics.Peking University,Beijing,100871)  
Nodal gene is critical to mouse development.It plays a vital role in the mesoderm formation and also has a biological function during mouse embryo pattern formation. To determine the basal promoter,the 5′ flanking sequence of Nodal gene were truncated and inserted into the luciferase reported vector. These constructs were transiently transfected into F?9 cells and the luciferase activity of each construct was determined. The basal promoter was located at about 60?bp up stream of the transcription initiation site. It contains a TATA box at -33?bp which is required for the transcription initiation. This basal promoter is active in many cell types,but its activity is not very strong.
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