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Cloning and Expression of the Rabbit Growth Hormone cDNA

DONG Yuqing\ LI Ronggui\ ZHAO Jindong\ WU Guangyao (College of Life Sciences,Peking University,Beijing,100871)  
The cDNA of preprotein and mature protein of rabbit hypophysiscerebri was obtained. The cDNA of mature growth hormone was cloned in pET\|3a, then the recombinant expression vector was transformed into E.coli BL21(DE3 plys).The overexpression was performed and the yield was 40% of the total protein.
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