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Cloning and Primarily Detecting the Function of the Promoter Region of NFL Gene in Tobacco

LIU\ Fuquan (College of Life Sciences,Peking University,Beijing,100871) ZHANG\ Yanwen (Department of Biology,Nankai University,Tianjin,30071) XU\ Zhihong (Chinese Academy of Sciences,Shanghai Institute of Plant Physiology,Shanghai,200032)  
The promoter region of NFL1 gene in tobacco was cloned by a novel PCR method,fused with the report gene GUS and transformed tobacco plants.The expression of GUS in the shoot apex of transgenic seedlings was detected.
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