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Identification and Expression of the Product from Engineering Bacterium Containing Recombinant Human Interleukin 18(IL-18) Gene

DONG Yuqing LI Ronggui\ DONG Chunxia\ ZHAO Jindong\ WU Guangyao (College of Life Sciences,Peking University,Beijing,100871)  
The cDNA of human Interleukin\|18 was obtained by RT\|PCR from the mRNA of human peripheral blood cells.The DNA sequencing was performed and the DNA was cloned into expression vector pBV220.The overexpression was performed in E.coli HB101.The expressive product was purified through Sephadex G100 and identified by N\|terminal amino acid sequencing and Western blot.
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