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《Journal of Beijing University of Chemical Technology(Natural Science Edition)》 2006-03
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Studies of the growth kinetics and induction strategy with a strain of recombinant yeast Pichia Pastoris expressing the human insulin gene

DONG Peng CHEN Jin-chun LENG Xue(College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029)  
The growth kinetics and induction strategy of a genetically engineered yeast Pichia Pastoris which expresses the human insulin gene have been studied.The results indicate that the relationship between the specific growth rate(μ) and the residual concentration of glycerol is in accord with the Monod equation in the cell growth phase where μ_(max)=0.204?h~(-1),and K_s=24.3?g/L.The maximum cell yield of glycerol is 0.497?g/g,and the growth maintaining coefficient m is 0.007?g/g·h.The latter value indicates that the strain has the potential for high density cultivation.It was found that the heterologous protein concentration could be increased by using a batch-fed yeast extract,with soyabean peptone and trace elements added in the induction phase.The heterologous protein concentration reached 256?mg/L in fermentation in a 10 L fermenter,which is 5 times the level obtained in flask fermentation.The results may be a guide to future industrial production.
【CateGory Index】: Q78;Q935
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