Correlation of Plasma Homocysteine Levels and Metabolic Enzyme Gene Polymorphisms with Cerebral Infarction
LI Bo;YAO Xiao-yang;Clinical Laboratory Shanghai Seventh People's Hospital;
Objective To explore the relationship between the Hcy and its main metabolic enzymes and cerebral infarction,to clarify the association between genetic factors and cerebral infarction in order to provide theoretical basis for clinical guidance. Methods We enrolled 60 patients with cerebral infarction and normal patients as cerebral infarction group and normal control group. Plasma Hcy levels were determined by HPLC,and the gene phenotype was determined by PCR-RFLP. Results Hcy level was significantly higher( P 0. 01). There were no significant differences when compared history of hypertension,hyperlipidemia and diabetes mellitus( P 0. 01) between two groups. MTHFR genotype of TT gene in the cerebral infarction group was significantly different( P 0. 01) when compared with the control group. The frequency of T allele was significantly higher than that of the control group( P 0. 01). There were significant differences in the frequency distribution of C677 T MTHFR genotype and allele( P 0. 01). The level of C677 T homozygous genotype MTHFR in Hcy gene was significantly higher than that in wild type( P 0. 01). There was a significant difference on plasma Hcy levels between CBS gene T27796 C homozygous genotype and heterozygous genotype( P 0. 01),which was significantly higher than that of wild type( P 0. 01).Logistic regression analysis showed a close relationship between plasma Hcy levels and C677 T MTHFR homozygous genotype in patients with cerebral infarction. Conclusion The level of Hcy is related to theincidence of cerebral infarction,which may be an independent risk factor for the occurrence of cerebral infarction. Homozygous mutations of MTHFR C677 T locus and T27796 C mutation in CBS gene may lead to increased plasma CY H level,which may be a genetic factor in the occurrence of cerebral infarction.
【CateGory Index】： R743.33