Clinical Significance of TNF-α,IL-6,CRP,Lp-PLA2,PON-1 and APN Value Changes in Pregnant Women with Preeclampsia
SHI Dan-dan;WANG Yong;GUO Jun-jun;WANG Na;BAI Yu-lai;Department of Obstetrics,Cangzhou Central Hospital;Department of Internal Medicine,Huanghua Shi Hospital of Traditional Chinese Medicine;
Objective To investigate the level of tumor necrosis factor-a,( TNFa),interleukin-6( IL-6),C-reactive protein( CRP),plasma lipoprotein associated phospholipase A2( Lp-PLA2),paraoxonase-1,( PON-1) as well as adiponectin( APN) in the irate pregnancy women with preeclampsia( PE),and to evaluate their clinical significance. Methods From August 2013 to June2016,160 cases of pregnant women were selected as research objects,which include gestational hypertension( GH)( n = 40),mild preeclampsia( MPE)( n = 40),severe preeclampsia( n = 40)( SPE) and normal pregnant woman( NC)( n = 40). The levels of TNF-a,IL-6,Lp-PLA2 were detected by Elisa( enzyme-linked immune sorbent assay) method. The level of CRP was measured by latex enhanced transmission immunoturbidimetry assay. The level of PON-1 and APN mRNA and protein were determined by qRT-PCR and Western blot respectively. Results The serum level of TNF-a,IL-6,CRP,Lp-PLA2 in PE pregnant woman were markedly elevated compared with normal group and gestational hypertension group. Meanwhile,for severe preeclampsia patients,their serum levels of TNF-a,IL-6,CRP,Lp-PLA2 were significantly higher than mild preeclampsia patients. Moreover,we found that the serum level of PON-1 and APN mRNA and protein in PE pregnant woman were markedly reduced compared with normal group and gestational hypertension group. Meanwhile,severe preeclampsia patient' s serum levels of PON-1 and APN mRNA and protein were significantly lower than normal group and gestational hypertension group. Conclusion Increased level of TNF-a,IL-6,CRP,Lp-PLA2 and decreased levels of PON-1 and APN could be involved in the pathogenesis of PE patients.
【CateGory Index】： R714.244