Effect of miR-137 on Proliferation and Apoptosis of Renal Carcinoma GRC-1 Cells
DONG Yan;XIAO You-wen;DONG Jian-hua;FANG Mei-rong;HU Xiang;LIU Yin;BAO Yong-qiang;PAN Hong-xia;LI Ting;HE Ling;Department of Nephrology,The People's Hospital of Leshan;
Objective To study the effect of miR-137 on proliferation and apoptosis of renal carcinoma GRC-1 cells. Methods miR-137 analogies were transfected into renal carcinoma GRC-1 cells. The non-transfection group( without transfection of miR-137 analogies),the miR-137 control group( transfected with random sequence) and the miR-137 transfection group( with transfection of miR-137 analogies) were used. The expression of miR-137 in cells was detected by fluorescence quantitative PCR in 48 h after transfection. The effect of miR-137 on proliferation and apoptosis of renal carcinoma GRC-1 cells was evaluated with the methyl-thiazolyl-tetrazolium( MTT) test,flowcytometry and immunofluorescence. Results 48 hours after the transfection of miR-137 analogies,fluorescence quantitative PCR detected that the relative expression levels of miR-137 in GRC-1 cells in the non-transfection group,the miR-137 control group and the miR-137 transfection group were( 24. 43 ± 2. 03) %,( 26. 57 ± 2. 55) % and( 73. 30 ± 3. 29) %,respectively( P 0. 05). MTT cell proliferation assay and flowcytometry showed that the proliferation rate of renal carcinoma GRC-1 cells was significantly lower and the apoptosis rate was significantly higher in the miR-137 transfection group than the non-transfection group and miR-137 control group at 48 hours after transfection( P 0. 05). However,there was no significant difference between the non-transfection group and miR-137 control group in the proliferation rate and apoptosis rate of renal carcinoma GRC-1 cells( P 0. 02).Conclusion miR-137 can inhibit the proliferation and promote the apoptosis of GRC-1 cells,which might be a newtarget for gene therapy of renal carcinoma.
【CateGory Index】： R737.11