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Enzyme-linked Immunosorbent Assay for Prostate Specific Antigen

Qin Fenghua;Zhong Weijiang;Chen Li; et al.(Institute of Radiation Medicine ,Academy of Military Medical Sciences ,Beijing 100850)  
Four hybridoma cell strains secreting monoclonal antibodies(McAbs QW1,QW4,QW7 and QW8) were obtained by immunizing mice with human PSA prepared in our laboratory.Hybridoma cell strains injected into the abdominal cavity of mice produced four McAbs with a titer of 10 ̄6,that of QW8 being the highest. Two-site sandwich enzyme-linked immunosorbent assay(ELISA) has been developed by the binding of the McAb coated on a microplate with biotinylated PcAb. Its sensitivity was 1. 0μg/L. The standard curve,accuracy and precision of the assay were found to comform to the quality control demands through the evaluation of serum dilution curve ,recovery rate and coefficients of variation. The mean of serum PSA in 10 patients with carcinoma of the prostate was 36. 5±2. 3μg/L ,significantly higher than that of the normal males.
【CateGory Index】: R392-33
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