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《LABELED IMMUNOASSAYS AND CLINICAL MEDICINE》 1994-01
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Enzyme-linked Immunosorbent Assay for Prostate Specific Antigen

Qin Fenghua;Zhong Weijiang;Chen Li; et al.(Institute of Radiation Medicine ,Academy of Military Medical Sciences ,Beijing 100850)  
Four hybridoma cell strains secreting monoclonal antibodies(McAbs QW1,QW4,QW7 and QW8) were obtained by immunizing mice with human PSA prepared in our laboratory.Hybridoma cell strains injected into the abdominal cavity of mice produced four McAbs with a titer of 10 ̄6,that of QW8 being the highest. Two-site sandwich enzyme-linked immunosorbent assay(ELISA) has been developed by the binding of the McAb coated on a microplate with biotinylated PcAb. Its sensitivity was 1. 0μg/L. The standard curve,accuracy and precision of the assay were found to comform to the quality control demands through the evaluation of serum dilution curve ,recovery rate and coefficients of variation. The mean of serum PSA in 10 patients with carcinoma of the prostate was 36. 5±2. 3μg/L ,significantly higher than that of the normal males.
【CateGory Index】: R392-33
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【References】
Chinese Journal Full-text Database 1 Hits
1 Shen Xia;Lu Yiwen;Shen Zheng;Zhu weiqi;Xin Hna Hospital;(Shanghai Second medicel University, Shanghai 200092);The clinical application of a quantitative assay of serum prostatic specific antigen[J];上海医学;1997-02
【Co-references】
Chinese Journal Full-text Database 2 Hits
1 Huang Yiran; Wu Haixiao; Yuan Jiming; et al(Department of Urology,Renji Hospital,Shanghai Second Medical University,200001);A study of prostatic specific antigen half-life in benign prostate hyperplasia[J];临床泌尿外科杂志;1996-04
2 Qin Fenghua;Zhong Weijiang;Chen Li; et al.(Institute of Radiation Medicine ,Academy of Military Medical Sciences ,Beijing 100850);Enzyme-linked Immunosorbent Assay for Prostate Specific Antigen[J];标记免疫分析与临床;1994-01
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