Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《LABELED IMMUNOASSAYS AND CLINICAL MEDICINE》 1994-01
Add to Favorite Get Latest Update

Measurement of Interleukin-6 by Sandwich ELISA with Monoclonal and Polyclonal Antibodies

Zhang Guoan; Liu Xiaopeng;Chen Zirong;et al.(Clinical Immunology Research Laboratory ,Air Force Fuzhou Hospital ,Fuzhou 350002)  
A sandwish ELISA for interleukin-6(IL-6) was developed by binding of anti-recombinant IL-6 monoclonal antibody coated on microplate with IL-6 in blood samples,which was detected by the addition of rabbit anti-rIL-6 serum and HRP-goat anti-rabbit IgG. Its interand intra-assay coefficients of variation were 3. 01% ̄8. 02% and 6. 84% ̄ 9. 04%, respectively and the recovery 96. 89% ̄99. 40%. The assay is easy to perform ,reproducible with a detection limit of 1ng/ml and can be used in both basic research and clinical investigation.
【CateGory Index】: R392-33
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 Zhang Guoan; Liu Xiaopeng; Chen Ziqun, et al.(Clinical Immunology Research Laboratory,Air Force Fuzhou Hospital, Fuzhou 350002);Interleukin-6 Level in Patients with Viral Hepatitis B and Its Significance[J];LABELED IMMUNOASSAYS AND CLINICAL MEDICINE;1995-01
2 Zhang Guoan~* Liu Xiaopeng Kang Dezhi. *Clinical Immunology Reasearch Laboratory,Fuzhou Air Force Hospital,Fuzhou 350002;Changes of interleukin-6 and platelet-derived growth factor in patients with malignant glioma[J];Chinese Journal of Medical Laboratory Sciences;1996-01
【Co-references】
Chinese Journal Full-text Database 1 Hits
1 Zhang Guo'an; Liu Xiaopeng;Xu Guoyan; Ji Yuan; Chen Zirong; Zheng Ying; Zhou Shanjian; Li Xiaoping( Department of Immunology Department of Medicine, Air Force Fuzhou Hospital, Fuzhou);Measurement of Plasma Cytokines Level in patients with Essential Hypertension and its Significance[J];Chinese Journal of Hypertension;1994-02
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 CHEN Ji-chao~*,XIAO Wei,ZHENG Chun-yan~*Department of Respiratory Medicine,the Aerospace Central Hospital,Beijing 100049,China;Effects of Astragalus on cytokine expressions of helper T lymphocyte subsets in peripheral blood mononuclear cells from lung cancer patients[J];Clinical Focus;2006-12
2 Zhang Guoan,Liu Xiaopeng,Chen Zirong,Shi Shuilan(Clinial Immunology Research Laboratory,The 476th Hospital of PLA,Fuzhou 350002);THE EFFECT OF THYMIC FACTOR D ON INTERLEUKIN6 LEVELS IN PILOTS[J];IMMUNOLOGICAL JOURNAL;1998-02
3 OUYANG Yao,DENG Fei,LI Chun_ming,LIU Hua_qing (The Affiliated Hospital of Zunyi Medical College,Zunyi 563003,China);THE EXPRESSION OF PLATELET DERIVED GROWTH FACTOR IN LUNG SQUMOUS CELL CARCINOMA AND ADENOCARCINOMA[J];Journal of Postgraduates of Medicine;2003-06
4 PAN De-sheng1, WANG Cheng-lin1, DIAO Hong-yu1, QI Jian-fei1,ZHAO Chong-zhi1,LIU Gui-jun2,SUN Dong-tao31. Department of Neurosurgery,The Second Affiliated Hospital,China Medical University,Shenyang 110004,China; 2. Department of Neurosurgery,Central Hospital,Huludao City; 3. Department of Neurosurgery,The Second Affiliated Hospital,Shenyang Medical College);Expression and Significance of Interleukin-6 mRNA in Gliomas[J];Journal of China Medical University;2003-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved