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Measurement of Interleukin-6 by Sandwich ELISA with Monoclonal and Polyclonal Antibodies

Zhang Guoan; Liu Xiaopeng;Chen Zirong;et al.(Clinical Immunology Research Laboratory ,Air Force Fuzhou Hospital ,Fuzhou 350002)  
A sandwish ELISA for interleukin-6(IL-6) was developed by binding of anti-recombinant IL-6 monoclonal antibody coated on microplate with IL-6 in blood samples,which was detected by the addition of rabbit anti-rIL-6 serum and HRP-goat anti-rabbit IgG. Its interand intra-assay coefficients of variation were 3. 01% ̄8. 02% and 6. 84% ̄ 9. 04%, respectively and the recovery 96. 89% ̄99. 40%. The assay is easy to perform ,reproducible with a detection limit of 1ng/ml and can be used in both basic research and clinical investigation.
【CateGory Index】: R392-33
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Chinese Journal Full-text Database 2 Hits
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Chinese Journal Full-text Database 1 Hits
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