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《Journal of Baoji University of Arts and Sciences(Natural Science Edition)》 2018-04
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Thermodynamic behavior of doxorubicin hydrochloride interacting with human serum albumin

WANG Jiao;LI Zong-xiao;College of Chemistry and Chemical Engineer,Shaanxi Key Laboratory of Phytochemistry,Baoji University of Arts and Sciences;  
Purposes—To study the thermodynamic behavior of the interaction between doxorubicin hydrochloride(DOX)and human serum albumin(HSA).Methods—Isothermal titration calorimetry,fluorescence spectroscopy UV spectroscopy and molecular simulation were used to investigate the aforesaid thermodynamic behavior.Result—The binding constants of DOX and HSA were 1.22×10~6,ΔH0,ΔS0;the fluorescence intensity decreased significantly,and the emission wavelength hardly moved.The highest integration of DOX in HSA site I and site II was 9.18 and 8.39,respectively.Conclusion—The interaction between DOX and HSA is electrostatic,and the quenching mechanism is static quenching,and the binding at site I is strong.
【Fund】: 陕西省教育厅项目(17JS009);; 陕西省高校科技青年人才提升项目协会(20170707);; 国家自然科学青年基金项目(51702006);; 宝鸡文理学院博士科研启动项目(ZK2017026);; 陕西省科技计划项目(2018JQ2056)
【CateGory Index】: R96
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