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《Chinese Journal of Blood Transfusion》 1989-04
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Determination of PKA Activity by Microtiter Plate Method

Cheng Yaqin, Yang Pengyun(The National Institute for Control Pharmaceutical and Biological Products)  
In the paper, a microtiter plate method was used to determine the residual prekallikrein activator(PKA) activity in blood products.The result of study indicated that it would be satisfactory that the reaction mixture of PKA and prekallikrein (PK) was iucul ?? at room temperature for 30 minutes with 0.05M Tris/0.15M NaCl buffer(pH 8.0) , and then the mixture continued to be incubated at room temperature for another 15 minutes after the chromogenic substrate for kallikrein was added. The method had a good reproducibility. The PKA contents determined in IVIG agreed with that of the processing method of IVIG and the hypotensive test of rats, and were basically consistent with the result, determined by the method developed by the Finnish Red Cross Blood Transfusion Service.
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【References】
Chinese Journal Full-text Database 2 Hits
1 Cheng Yaqin,(The National Institute for Control of Pharmaceutical and Biological Products);Extraction of Prekallikrein Activator[J];Chinese Journal of Blood Transfusion;1990-01
2 Zhou Yong National Institute for the Control of Pharmaceutical and Biological Products;Determination of C 1-Inhibitor Activity by method of Inhibition to Prekallikrein Activator[J];Chinese Journal of Biologicals;1991-03
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