Testing the Human Serum for HBsAg by Ultrasensitive Chemiluminescent Photographic Detection-Dot-ELISA Technique
Liu Xiaoyu, (Institute of Blood Transfusiono. Chinese Academy of Medical Sciences)
The most new ultrasensitive chemiluminescent photographic detectiondot-ELISA (CPD-Dot-ELISA) technique was developed by combining the chemiluminescence technique with Dot-ELISA. The sensitivity of testing for pure HBsAg by CPD-Dot-ELISA was 30 and 60 times higher than by general Dot-ELISA and plate ELISA, respectively. 243 clinical serum specimens had been tested for HBsAg by both plate ELISA and CPD-Dot-ELISA, indicating that of 243 specimens tested for HBsAg by the former, 149 were positive, while of 243 specimens tested for HBsAg by the latter, 194 were positive. Of 149 positive specimmens tested by the former, only Ⅰ wasn't detected by the latter. 14 specimens randomly sampled from the additional 45 positive serum specimens detected by the latter, and 2 serum specimens which proved to be positive by both methods, had then been subject to neutralized test for HBIG, indicating that all 16 mentioned above specimens were positive. The results showed that the CPD-Dot-ELISA technique not only had its high sensitivity, good specificity and repro-ducibility, but also it was simple in manipulation, economically practical and worthy to be widely spread.