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《Chinese Journal of Blood Transfusion》 2007-01
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Sequences analysis for a novel HLA-B allele: HLA-B~*4608

HE Junjun, Lv Qinfeng, ZHANG Wei, et al. Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, China  
Objective To analyze the nucleotide sequences of novel allele HLA-B*4608. Methods Genomic DNA of the proband was extracted from whole blood by the commercial DNA extraction kit. The HLA-B exons 1-8 of the proband was amplified and the amplified product was cloned by TOPO TA cloning sequencing kit to split the two alleles apart. Both strands of exons 2, 3 and 4 of chosen colonies were sequenced. Results The sequencing results showed HLA-B alleles of the proband as B*5502 and a new allele as proved by blast search. The sequences of the novel allele have been submitted to Genbank(DQ177521,DQ177522,DQ177523). The new allele is similar to HLA-B*4601 except for two nucleotide substitutions in exon 3: T to C at nucleotide position 538 and G to T at nucleotide position 539. These result in an amino acid substitution at codon 156 from W to L. Conclusion This allele is a novel allele and has been officially named B*4608 by the WHO Nomenclature Committee.
【CateGory Index】: R446.6
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 ZHU Fa-ming,LU Qin-feng, ZHANG Wei,ZHANG Hai-qin,FU Q i-hua,YAN Li-xing. (Blood Center of Zhejiang Province,Hangzhou,Zhejiang,310 006 P.R.China);Identification and sequence analysis of a novel HLA-B*5614 a llele[J];Chinese Journal of Medical Genetics;2005-03
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 ZHANG Wei 1,2 ,L Qin-Feng2,WANG Wei2,HAN Zhe-Dong2, ZHU Fa-Ming2,YAN Li-Xing2 1Institute of Immunology, Medical College of Zhejiang University, Hangzhou, 310006, China; 2Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research, Ministry of Health,Hangzhou 310006, China;Sequence Analysis of HLA-B* 4061 Allele Newly Found[J];Journal of Experimental Hematology;2006-06
2 ZHANG Wei, HE Jun-Jun, L Qin-Feng, WANG Wei, HANG Zhe-Dong, ZHU Fa-Ming, YAN Li-XingBlood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, China;Sequence Analysis of A Novel HLA-A*2459 Allele[J];Journal of Experimental Hematology;2007-05
3 ZHU Fa-Ming, JIANG Kan, L Qin-Feng, ZHANG Wei, HE Ji, XU Xian-Guo, YAN Li-XingBlood Center of Zhejiang Province, Key Laboratory of Blood Safety Research, Ministry of Health. Hangzhou, 310006, China;Analysis of Natural Killer Cell Immunoglobulin-like Receptor Gene Family in HLA-identical Sibling[J];Journal of Experimental Hematology;2007-06
4 DAI Wei-Jian1, ZHANG Wei2, HE Jun-Jun2, WANG Wei2, HAN Zhe-Dong2, ZHU Fa-Ming2, YAN Li-Xing1,21The First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310003, China; 2Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, China;Identification and Sequence Analysis of a Novel HLA-B*3936 Allele[J];Journal of Experimental Hematology;2007-06
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 ZHU Fa-ming, XU Xian-guo, HONG Xiao-zhen, YAN Li-xing. (Blood Center of Zhejiang Province,Hangzhou,Zhejiang,310006 P.R.China);Two base deletion of the alpha(1,2) fucosyltransferase gene responsible for para-Bombay phenotype[J];Chinese Journal of Medical Genetics;2004-03
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