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《Chinese Journal of Blood Transfusion》 2007-01
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~(137)Cs gamma irradiation for sterilization of red blood cells contaminated by escherichia Coli

LI Ruqing,JIANG Tianlun,XIAO Ruiqing,et al. Department of Blood Transfusion,Southwest Hospital,The Third Military Medical University,Chongqing 400038,China  
Objective To study the bacteriological effect of different dosages of gamma irradiation on Escherichia Coli (E. Coli) in red blood cells (RBC) products,and assess the appropriate dose of gamma irradiation. Methods Prepare RBC samples inoculated with(102-106)/ml of E. Coli. The contaminated RBCs were irradiated by 0 (as control),15,20,25,30 and 35Gy of gamma irradiation. E. Coli in the RBCs were tested on days 0,7, and 14 after irradiation. Results The quantities of E. Coli changed after irradiation. The RBCs inoculated with less than 103cfu/ml of E. Coli were completely sterilized by 25 Gy irradiation. Conclusion 25 Gy gamma irradiation can eradicate less than 103cfu/ml of E. Coli in the RBC products.
【CateGory Index】: R457.1
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【Secondary References】
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1 LI Lan,ZHANG Qiong-li,XUE Jian-cheng(Department of Blood Transfusion,Second People's Hospital of Shenzhen,Shenzhen 518035,China);Observation on the Clinical Application of ~(137)Cs Gamma-ray Irradiated Blood Component in Hemopoietic Stem Cell Transplantation[J];Journal of Clinical Research;2008-12
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