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《Chinese Journal of Blood Transfusion》 2007-06
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Morphology changes and the expression of phosphatidylserine in stored platelets

XIONG Wen,ZHU Zhankun,WU Xuqun,et al.Transfusion Medicine Institute,Shenzhen Blood Center,Shenzhen,Guangdong 518035,China  
Objective To study the morphology changes and the expression of phosphatidyl serine(PS) in apheresis platelets(Plts) during storage,also to analyze their relationship,in order to provide laboratorial evidence for the in vitro storage period of plts.Methods The morphological changes of plts during 8-day storage were detected by May-Grunwald-Giemsa dyeing method,and the expression of PS on platelet membrane was tested by flow cytometry(FCM).Results The morphological lesions appeared on the fourth day of storage,because the modified morphological score(MMS) of 4-day group was significantly lower than that of fresh plts(t=2.341,P0.05).The MMS kept on decreasing with extended storage,and on the seventh day of storage,the MMS declined to 31%.However,the PS expression rate of 1-day storage group was obviously higher than that of 0-day group(t=3.088,P0.05).The PS expression showed no changes during 1-3 day storage,but increased markedly on the fourth day(t=2.1612,P0.05).During 5-8 day storage,the PS expression kept on increasing,and there were significant differences among groups(all P0.05).A negative correlation between MMS and PS expression rate was shown in stored plts(r=-0.9923,P0.01).Conclusion There is a significant negative correlation between MMS and PS expression rate in stored Plts.The MMS index of stored Plts suggests that the storage time for apheresis Plts could be experimentally prolonged to 7 days.
【CateGory Index】: R457
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