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《Chinese Journal of Blood Transfusion》 2007-06
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Influence of time and temperature on Rh antigen masking by maleimidophenyl-polyethylene glycol

LI Zhiru1,ZHOU Ying1,ZHANG Rong2,YANG Qunshen,et al.1.Chengdu Blood Center,Chengdu,Sichuan 610081,China;2.College of Chemistry,Sichuan University  
Objective To study the effect of temperature and time on the mask of Rh antigen,during the surface decoration of RBC,by maleimidophenyl-polyethylene glycol(MDPEG).Methods RBCs(about 3-5%) were decorated with MDPEG(30mg /ml) and 2-iminothiolane(3mg/ml) in buffer(pH 7.4),and then tested with anti-A,anti-B and anti-RhD.The agglutination of PEG-RBC with anti-RhD was observed with light microscope,and the effect of RhD antigen mask was evaluated.Results The time order of adding MDPEG,2-iminothiolane and RBCs had no significant effect on the Rh antigen mask,while the temperature and time did matter.Decorated at 25℃-37℃ for 30 minutes could completely mask the RhD antigen,while at 4℃ for 120 minutes could only partly mask the RhD antigen,and some small RBC agglutinates could still be observed with microscope.Conclusion MDPEG linking to RBC membrane catalyzed by 2-iminothiolane,to mask RhD antigens on RBCs was a chemical reaction.During the decoration,temperature and time have an important effect on the RhD antigen mask,while the time order of adding MDPEG,2-iminothiolane and RBC does not matter.
【CateGory Index】: R392
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【References】
Chinese Journal Full-text Database 1 Hits
1 LI Zhi-ru,YANG Qun-shen,SONG Ling,et al.(Chengdu Blood Center,Chengdu 610081,China);STUDY ON CONCEALMENT EFFECT OF MALEIMIDOPHENYL POLYETHYLENE GLYCOL ON RHD ANTIGEN OF STEALTHY RED CELLS[J];Modern Preventive Medicine;2009-03
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Ji Shouping,Liu Zepeng,Ren Huiming,et al.Field Institute of Blood Transfusion,Academy of Military Medical Sciences.;Preliminary Study on Preparation of Universal Donor Blood by Camouflage of Red Blood Cell Surface Antigens with Methoxy Polyethylene Glycol[J];Chinese Journal of Blood Transfusion;2000-04
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Jiexi,Liu Zhepeng,Cao Qiong,et al.Institute of Blood Transfusion,Academy of Military Medical Sciences,Beijing 100850,China;Chemical camouflage of like human B antigens on Rhesus monkey red blood cells with methoxy polyethylene glycol[J];Chinese Journal of Blood Transfusion;2003-04
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8 LI Su-Bo, JIA Yan-Jun, LIU Ze-Peng, ZHANG Yang-Pei (Institute of Transfusion Medicine,Academy of Military Medical Sciences, Beijing 100850, China);Determining half life of mPEG-sheathed erythrocyte with FITC labeling[J];Bulletin of The Academy of Military(Medical Sciences);2003-05
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【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 Ji Shouping,Liu Zepeng,Ren Huiming,et al.Field Institute of Blood Transfusion,Academy of Military Medical Sciences.;Preliminary Study on Preparation of Universal Donor Blood by Camouflage of Red Blood Cell Surface Antigens with Methoxy Polyethylene Glycol[J];Chinese Journal of Blood Transfusion;2000-04
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3 Li Fengzhen;Sun Manchi Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences;MICROCOLORIMETRIC ASSAY OF CHOLINESTERASE ACTIVITY[J];Bulletin of The Academy of Military(Medical Sciences);1986-03
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