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《Chinese Journal of Blood Transfusion》 2012-01
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Preliminary research on the preparation and function evaluation of the endotoxin adsorption materials

LIU Xi1,LU Yong2,LV Fuhong2,WANG Hong1,LIU Jiaxin1.1.Institute of Blood Transfusion of Chinese Academy of Medical Sciences,CAMS & PUMC,Chengdu 610052;2.Sichuan Union Bio-technology Ltd. Company  
Object To prepare the endotoxin adsorption materials by using Poly(vinyl alcohol)(PVA) as the carrier and different Amines as affinity ligands and then proceed to evaluate their adsorption capability and blood compatibility.Method PVA was graft-polymerized respectively by ethylendiamine(PN-1),hexane diamine(PN-2),triethylene tetramine(PN-3) and tetraethylene pentamine(PN-4) to obtain four different adsorption materials;the amount of free-amine and nitrogen can be measured.The water adsorption capability was accessed.The static adsorption equilibrium time and the static adsorption rate in water were measured by tachypleus amebocyte lysate(TAL) and then analysed.We also evaluated the blood compatibility of these adsorbents through hemolysis test and non-specific adsorption test of plasma total protein and albumin.Result The amounts of free-amine in PN-1,PN-2,PN-3 and PN-4 were 353.49 μmol/g,419.72 μmol/g,644.72 μmol/g and 880.67 μmol/g,nitrogen were 378.57 μmol/g,521.42 μmol/g,1071.42 μmol/g和1628.57 μmol/g.The water adsorption rate of PN-2 was reaching 179.03% in 1h,much higher than that(89.15%) of the pure PVA.The static adsorption equilibrium times were all about 60min at which the static adsorption rate of PN-1was 75.0% while that of PN-2,PN-3 and PN-4 were 94.4%,90.0%,95.5% respectively.The hemolysis rates of PVA,PN-1,PN-2,PN-3,PN-4 were respectively 0.4%,0.7%,0.01%,0.4%,0.2%.The recovery rates of Total Protein were respectively 82.50%、98.11%、98.61%、96.21%、91.10%.The recovery rates of Albumin were respectively 78.82%、92.19%、92.73%、90.48%、88.24%.Conclusion The four different affinity materials consisting of PVA as the carrier and different Amines as affinity ligands,show good endotoxin adsorption rate in water.Their hemolysis rates are all below the national standard limit(5%) and their non-specific adsorption of plasma total protein and albumin are minimal.PN-2 performs better in all aspects.
【CateGory Index】: R318.08
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