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《Chinese Journal of Blood Transfusion》 2014-01
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The quality of automatic extracted DNA associated with blood sample status

Ll Wangxia;LIU Guangjian;LU Huax-in;SHEN Gang;ZHU Yuanyan;ZOU Jie;Wuhan Blood Center;  
Objective To explore the related factors for a better DNA extraction,such as anticoagulation status,time of cold storage,and refreezing times before extracting DNA from whole blood.Methods All whole blood specimens were anticoagulate by EDTA.And all specimens were grouped by three factors mentioned above.The groups were classified as fully anticoagulating group(n = 231),half anticoagulating group(n = 59),fresh cold storage group(n = 231,- 20℃,within 7 days),fresh refrigerated group(n = 62,4℃,within 7 days),obsolete refrigerated group(n = 62,4℃,more than 24 days),and repetitive freeze-thawing group(n = 32).DNA was extract by automated instrument base on magnetic beads method.DNA concentration and purity were detected,and then submitted to statistical analysis.Results The DNA concentration and purity of full anticoagulating group is higher than half anticoagulating groups.Meanwhile,the DNA concentration and purity is also going down along with fresh refrigerated group,fresh frozen group,and obsolete refrigerated group.Except for no significant decline between two and three times freeze-thawing,the DNA concentration and purity is going down when samples refreeze more times.Conclusion Half anticoagulating,long refrigerating time and too much freeze-thawing all affect DNA concentration and purity,while anticoagulation and refrigerating time contribute the most.
【CateGory Index】: R457.1
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