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《Chinese Journal of Blood Transfusion》 2014-01
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Comparative analysis of two methods for detection of red cell alloantibodies

YAN Dagang;CHEN Yinghong;DONG Yuhong;DUAN Chunyan;Panzhihua Central Hospital;  
Objective:To compare the potency of red blood cell alloantibody testing between the microtube column gel antiglobulin test and indirect antiglobulin test(IAT) method.Methods Red blood cell alloantibodies,from 148 cases were examined in parallel by the tube IAT and microcolumn gel antiglobulin test.Results There were 98.0% samples to be detectable with microcolumn gel antiglobulin method and accuracy was 81.8% to IAT method.The potency of detection of clinically significant alloantibodies varied between the test systems:in tube IAT,all serums(plasmas) of antibody to-D,-E,-C,-c,-e,-C + e,-E + c,-N,-S,-s,-Dia,-Fya,-Fyb,-Jka,-Jkb,-K,-k,-Lua,-Lub,-Lea,-Leband-P1 were detected.But only 36.0% serums contained anti-M were detected and 56.0% serums contained cold alloantibody were detected.In the microtube column gel antiglobulin test,all serums of antibody were detected except Kidd system.A rather high number(37.5%) of antibodies were missed in gel test.Conclusion The potency of gel test was higher than IAT in the detection of alloantibodies with clinical significance,but alloantibody of Kidd system would be missed in the gel test.A proposal has been that both methods should be performed in routinely to the unmatched blood samples.
【CateGory Index】: R446.6
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1 Shen Aiwen,Su Jinai.Conghua Blood Bank of Guangzhou Blood Center,Conghua 510900,Guangdong,China;The value of micro-column gel Coomb′s test in the diagnosis of hemolytic diseases[J];Guangzhou Medical Journal;2010-03
2 LI Cun-yan,ZHENG Shu-juan,.the People’ Hospital of Hunan Province, Changsha 410005, Hunan, CHINA;Comparison of gel microcolumn assay with tube agglutination test and polybrene test in detection of blood plasma weak blood group antibody[J];Hainan Medical Journal;2010-12
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