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《Chinese Journal of Blood Transfusion》 2014-01
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The application of the polybrene microplate in the irregular antibody screening

HE Mingdi;XU Tingting;ZHANG Lijie;JIANG Mengtian;REN Ming;SHEN Gang;Wuhan Blood Center;  
Objective The polybrene microplate,a standard method for blood donors' irregular antibody screening was established.Methods The IgG anti-D anthropogenic serum was selected,which their titer was 16.Based on traditional polybrene tube method,researched the influence that different volumes of serum and LIM on red blood cell agglutination by the orthogonal principle,and determined the optimum parameters of shock through the observations.Simultaneously,the sensitivities of microplate method and Polybrene tube method were compared.Results The specifity of polybrene microplate method is 96.87% and its sensitivity is 98.44% by using the optimum reaction system,that centrifuging 1000 r / min,shocking 10 s,50 μL serum,150 μL LIM.Conclusion We developed a novel antibody screening method,which can instead of the traditional Polybrene tube method,it also could be batch operated and beautomatedeasily.
【Fund】: 武汉市卫计委临床医学科研项目(WX13D08)
【CateGory Index】: R446.6
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 CAO Kuijie 1 HU Lihua 1 LIU Feng 1 ( 1Department of Blood Transfusion,Union Hospital,Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022);Detection of irregular antibodies in clinical blood transfusion[J];Journal of Clinical Hematology;2002-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 DENG Mei-ying1,CHEN Yu1,WANG Jin-heng1,et al.(1.Department of Blood Transfusion,Affiliated Hospital of Guilin Medical College,Guilin,Guangxi 541001,China;2.Central Blood Bank of Guilin,Guangxi 541001,China);Clinical observation of irregular antibodies detection of 6036 patients with blood transfusion[J];Chongqing Medicine;2010-07
2 Chen Yong;Wang Yucheng;Chen Chen;Chen Fei;Department of Clinical Laboratory Blood Bank,Brain Hospital Affiliated to Nanjing Medical University;;Significance of irregular antibody screening before blood transfusion in clinical blood transfusion[J];International Journal of Laboratory Medicine;2013-19
3 HUA Minyu;JIANG Jian;HONG Jun;Wuxi Red Cross Blood Center;;Application of the irregular antibody detection in prediction of non ABO hemolytic disease of the newborn[J];Chinese Journal of Blood Transfusion;2014-03
4 MA Hong-wei,CHENG Si-guo,BIE Li-li,CHEN Zan,JIANG Yu,DONG Xu,QIU Cai-li(Henan Red Cross Blood Center,Zhengzhou 450012,China);Phenotype and RHD gene analysis of Del in Rh negative individuals of Henan[J];Henan Medical Research;2009-04
5 ZHENG San,FENG Xue-guan(Transfusion Department,Affiliated Hospital to Hainan Medical College Haikou 570102,China);Clinical application of micro-column gel agglutination assay in testing irregular antibody[J];Journal of Hainan Medical College;2009-10
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7 PAN Ji-chun,YU Yang(Department of Blood Transfusion,General Hospital of PL A,Beijing 100853,P.R.China);Evaluation on Crossmatching with Modified Slide Polybrene[J];Science Technology and Engineering;2007-20
8 LI Lan,ZHANG Qiong-li,DONG Hong-qiang(Department of Blood Transfusion,the Second People's Hospital of Shenzhen,Shenzhen 518035,China);Clinical Significance of Screening and Identification of Irregular Antibodies in Patient Serum[J];Journal of Tropical Medicine;2008-11
9 YIN Wen-jie1,RUAN Chang-geng2(1.College of Life Science,Fudan University,Shanghai 200433,China;2.College of Medicine,Soochow University,Suzhou 215123,China);Recent Research and Prospect on Blood Group Antigen Modification on Erythrocytes[J];Journal of Soochow University(Engineering Science Edition);2007-01
10 LI Lan,WU Chang-lin,DANG Xin-tang,DONG Hong-qiang,ZHU Yi(Department of Blood Transfusion,The Second People's Hospital of Shenzhen,Shenzhen 518035,Guangdong Province,China);Clinical Significance of Screening of Rh-negative Blood Group and Detection of Irregular Antibody[J];Chinese Journal of Biologicals;2011-05
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