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《Chinese Journal of Blood Transfusion》 2014-01
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The application of the polybrene microplate in the irregular antibody screening

HE Mingdi;XU Tingting;ZHANG Lijie;JIANG Mengtian;REN Ming;SHEN Gang;Wuhan Blood Center;  
Objective The polybrene microplate,a standard method for blood donors' irregular antibody screening was established.Methods The IgG anti-D anthropogenic serum was selected,which their titer was 16.Based on traditional polybrene tube method,researched the influence that different volumes of serum and LIM on red blood cell agglutination by the orthogonal principle,and determined the optimum parameters of shock through the observations.Simultaneously,the sensitivities of microplate method and Polybrene tube method were compared.Results The specifity of polybrene microplate method is 96.87% and its sensitivity is 98.44% by using the optimum reaction system,that centrifuging 1000 r / min,shocking 10 s,50 μL serum,150 μL LIM.Conclusion We developed a novel antibody screening method,which can instead of the traditional Polybrene tube method,it also could be batch operated and beautomatedeasily.
【Fund】: 武汉市卫计委临床医学科研项目(WX13D08)
【CateGory Index】: R446.6
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Chinese Journal Full-text Database 1 Hits
1 CAO Kuijie 1 HU Lihua 1 LIU Feng 1 ( 1Department of Blood Transfusion,Union Hospital,Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022);Detection of irregular antibodies in clinical blood transfusion[J];Journal of Clinical Hematology;2002-04
Chinese Journal Full-text Database 10 Hits
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