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《Chinese Journal of Blood Transfusion》 2014-04
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Evaluation and validation of the applicability of parameters on Beckman PK7300 automatic blood analysis system

JIA Junjie;WANG Rui;LIU Yaqing;CHEN Yu;JIANG Ying;GE Hongwei;Laboratory Medicine,Beijing Red Cross Blood Center;  
Objective To evaluate and validate the applicability of the parameters on Beckman PK7300 automatic blood analysis system in the blood screening laboratory.Methods Accroding to the recommends of manufacturers and other laboratories,we determined 6 parameters including incubation temperature,reagent volumn,sample diluents ratio,diluted sample volumn,determination parameters and so on.The other 3 parameters(including incubation time,anti-A and anti-B dilution of standard serum,dilution multiple of sample serum) were evaluated with orthogonal experimental principle.The sensitivity and specificity of detecting system were evaluated and validated by using of conventional and special blood type samples.Results Using the evaluated parameters,the 2 426 samples which known ABO blood group were done forward typing and reverse typing detection.The rate of correct interpretation was 99.92%.Simultaneously,the 10 cases of A2B subtype were detected correctly.Conclusion It indicated that the sensitivity and specificity of detecting system can meet the relevant requirements,moreover it can effectively improved the processing ability and economic benefits.
【Fund】: 卫生部卫生行业科研专项资助项目(201002005)
【CateGory Index】: R446.6
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