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《Chinese Journal of Blood Transfusion》 2014-04
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The prevalence of human bocavirus in partial Chinese plasma derivatives

LI Hongxue;LI Wuping;Institute of Blood Transfusion,Chinese Academy of Medical Sciences & Peking Union Medical College;Institute of Pathogen Biology,CAMS & PUMC;  
Objective To offer informations on the genomic and sero-prevalence of human Bocavirus(HBoV) in partial Chinese plasma derivatives.Methods Based on the ABI 7 900 HT Fast real-time PCR system platform,we established a specific and sensitive real-time PCR(SYBR) assay to detect HBoV DNA.On the basis of method constructed above,we tested 326 plasma derivatives[including 65 batches of albumin,197 batches of intravenous immunoglobulinfrom(IVIG),47 batches of anti-hemophilic factor Ⅷ(FⅧ),12 batches of Fibrinogen(Fg),5 batches of Prothrombin Complex Concentrate(PCC) ] collected from 3 Chinese blood product manufacturers between 2009 and 2012 for the genomic prevalence of HBoV in different kind of plasma derivatives in China.Nested PCR were performed to confirm the positive samples tested by real-time PCR.HBoV DNA positive samples were also screened for antiHBoV-IgG and IgM to obtain the status of HBoV antibodies.Results A total of 44 samples were HBoV genome positive in all the plasma derivatives tested in our study,the positive rate is 13.50%.HBoV DNA was detected in 3.08%(2 /65) albumin samples,7.11%(14 /197) IVIG samples,55.32%(26 /47) anti-hemophilic factor Ⅷ samples,16.67%(2 /12) fibrinogen samples,respectively,no HBoV DNA was detected in the five PCC samples.Among all the positive samples,2 FⅧ samples were quantified with HBoV viral loads over 104 copies /mL.As a result,33 genomic positive samples were tested for HBoV antibodies,all were negative for anti-HBoV-IgM,33.33%(11 /33) were positive for anti-HBoV-IgG.Conclusion HBoV are detectable in Chinese plasma derivatives at low levels.
【CateGory Index】: R446.11
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