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《Chinese Journal of Blood Transfusion》 2014-04
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Molecular genetics study on rare B(A) 04 phenotype

HAN Bin;FENG Zhihui;YAN Ping;JIAO Shuxian;Institute of Transfusion Medicine,Qingdao Blood Center;Clinical Laboratory,Qingdao Haici Hospital;  
Objective To determine the ABO genotypes of the blood samples which serological results seem to like B(A) by DNA sequencing analysis.Methods Seven blood samples which is difficult to determine the group by the serological method were preliminary genotyped by PCR-SSP.Complete regions of exon 6 and 7 in the ABO gene were amplified by PCR and the products were directly sequenced to determine the genotype,and also identified its allele.Results The serological results of seven blood samples were similar:red cells were weakly agglutinated by anti-A,strongly agglutinated by anti-B,not agglutinated by anti-A1,and the serum contained anti-A.They were preliminarily genotyped as B/O1 by PCR-SSP.Results of the direct and haplotype sequencing showed that the seven samples contained an O allele,and the B alleles are present nt 640A G mutation in exon 7,confirming it as B(A) 04 allele.Conclusion It was confirmed thatthe seven samples were B(A) phenotype and carrying B(A) 04 allelethrough analysising the sequence and serological results
【CateGory Index】: R440
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【Citations】
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