Effects of Yinchenhao decoction on TNF-α level of BALB /c mouse immunized with A blood group antigen

LI Hui;YU Mingming;YAN Qingya;YU Yantao;CHAO Yan;ZHANG Jianyuan;LIU Chixiang;SHI Wen;ZHOU Huayou;Guangdong Provincial Hospital of Chinese Medicine,the Second Affiliated Hospital of Guangzhou University of Chinese Medicine;Shunde Central Blood Station;  

Objective To research effects of Yinchenhao decoction on secretion of tumor necrosis factor-α( TNF-α) in mice immunized with ABO blood group antigens and to investigate the correlation of TNF-α level with the changes of anti-A. Methods Taking BALB/c mice immunized with human erythrocytes of A blood group as a model,the TNF-α in BALB/c mice plasma was detected with ELISA,and the IgG-typed anti-A of BALB/c mice was assayed with indirect antiglobulin test( IAT) as well as absorption and elution test. The correlation of TNF-α level with the changes of IgG-typed anti-A,in immune group treated with A-type erythrocytes and therapy group treated with Yinchenhao decoction,was investigated. Results TNF-α in control group,A-type erythrocyte immune group and Yinchenhao decoction treatment group were( 83. 50 ± 6. 32) ng/L,( 135. 56 ± 44. 14) ng/L and( 109. 44 ± 12. 50) ng/L,respectively. There existed significant differences between groups( P 0. 05),and showed all P 0. 05 by multiple comparisons. The effective rate in Yinchenhao decoction treatment group was 61. 11%,and there showed no significant differences of TNF-α levels in effective group than in ineffective group. There showed a significant positive correlation between the TNF-α levels and the changes of anti-A( P 0. 05). Conclusion BALB/c mouse study suggested that Yinchenhao decoction can inhibit the secretion of TNF-α and ABO blood group antibodies,and there showed a significant positive correlation between the levels of TNF-α and the changes of anti-A.

【Fund】： 广东省科技计划项目(2010B031600267);; 广州中医药大学创新基金(2007C072)
【CateGory Index】： R285.5

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