Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Blood Transfusion》 2014-06
Add to Favorite Get Latest Update

The effects of N-Arachidonoylethanolamine on cytochrome C release and apoptosis of storage platelets

ZHANG Yunlong;LI Peng;LIU Yan;ZHANG Yi;QIAO Wenben;YU Yuan;FANG Yunhai;XU Qun;ZHU Chuanfu;ZHANG Wenjing.;Blood Center of Shandong Province;  
Objective To evaluate the effect of NArachidonoylethanolamine( ANA) on cytochrome C( Cyt-C)release and apoptosis in platelets( PLTs). Methods Apheresis PLTs taken from donors were split into two parts. An aliquot of0. 5 μmol /L ANA final concentration was added to one part of storage PLTs as the experimental group( n = 20),the other with-out ANA as the control group( n = 20). The two groups samples were stored on a flat-bed shaker at( 22 ± 2) ℃ up to 7days. The expression of phosphatidyl serine( PS) positive was determined by flow cytometer. The expressions of Cyt-C and caspase-3 were detected by Western blotting. And caspase-3 activity was measured by ELISA method. Results Comparing the experimental group with the control group,the rates of PLT PS positive were( 8. 29 ± 1. 44) % vs( 14. 24 ± 2. 47) %( P 0. 05); the expressions of activated caspase-3 were( 0. 063 ± 0. 014) % vs( 0. 132 ± 0. 025) %( P 0. 05); the activities of caspase-3 were 0. 096 ± 0. 04 vs 0. 208 ± 0. 03( P 0. 05); the releases of Cyt-C from mitochondria to cytosol were( 3. 18± 1. 32) % vs( 15. 13 ± 3. 40) %( P 0. 05). Conclusion ANA can inhibit cytochrome C release into the cytoplasm,and reduce caspase-3 activity and PLT apoptosis. This may be one important mechanism of protecting PLT from apoptosis by ANA inhibiting mitochondria-mediated pathway.
【Fund】: 山东省优秀中青年科学家科研奖励基金(BS2011SW044);; 山东省医药卫生科技发展计划重点项目(2013WS0170)
【CateGory Index】: R446.11
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 CHEN Jian-yu;LIN Bing;ZHANG Hong;QIN Lu-ping;School of Pharmacy, Fujian University of Traditional Chinese Medicine;School of Pharmacy, The Second Military Medical University;;Research progress on traditional Chinese medicine for controlling cytochrome C[J];Drugs & Clinic;2014-03
2 CHEN Hui-jun;YANG Yang;Department of Pediatrics,Xinxiang Central Hospital;Nanfang Hospital in Southern Medical University;;Study on protective effect and its mechanism of pretreatment of Caspase-3 gene inhibiter on hypoxic-ischemic brain damage in the neonatal rats[J];Chinese Journal of Child Health Care;2014-01
3 CAO Dongming;LI Xiaohui;LI Long;PEI Haitao;Department of Emergency Neurology,The Affiliated Hospital of Medical College,Qingdao University;Department of Urology,Zhongshan Hospital,Fudan University;;Protective Effect of Bone Morphogenetic Protein-7 on Focal Cerebral Ischemia-Reperfusion Injury in Rats[J];Chinese Journal of Clinical Medicine;2013-05
4 Li Qiang;ZHAI Yu;ZHANG Ting;CHEN Rong-fu;SUN Xiao-jiang;Department of Neurology, Shanghai Sixth People’s Hospital, Shanghai Jiaotong University;Department of Neurology, the Ninth People’s Hospital, School of Medicine, Shanghai Jiaotong University;;Dynamic Characteristics of Mitochondria Damage Induced by Oxidative Stress after Cerebral Ischemia Reperfusion[J];Chinese Journal of Clinical Neurosciences;2014-03
5 WANG Xiao-ping;LUO Yong-jie;MA Si-wen;LIU Jun-xia;Department of Neurology,Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital;Graduate School of Luzhou Medical College;;Dynamic expressions of Bcl-2 and Caspase-3 in acute cerebral infarction rat model[J];Practical Journal of Clinical Medicine;2013-05
6 Gao Chen;Zhou Haiyan;Li Zhiyun;Wang Xiangyang;Jia Pin;Zhang Jingke;Department of Neurosurgery,Anning Branch Hospital, Lanzhou General Hospital of Lanzhou Command;Department of Ophthalmology,the First Affiliated Hospital of Lanzhou University;Department of Neurosurgery,Lanzhou General Hospital of Lanzhou Command;Center of Laboratory Animals,Lanzhou University;;Content of reactive oxygen species in and apoptosis of retinal ganglion cells in rats with cranio-orbital injury[J];Chinese Journal of Minimally Invasive Neurosurgery;2013-10
7 ZHAO Li-Li;CHEN Meng-Xing;ZHANG Ming-Yi;DAI Ke-Sheng;Laboratory of Hematology and Cell Biology,School of Biological Science and Medical Engineering,Beijing University of Aeronautics and Astronautics;Jiangsu Institute of Hematology,Key Laboratory of Thrombosis and Hemostasis,Ministry of Health,The First Affiliated Hospital of Soochow University;;Protein Kinase C Activation Induces Platelet Apoptosis[J];Journal of Experimental Hematology;2013-05
8 ZHUANG Yun-Long;ZHANG Yi;QIAO Wen-Ben;YU Yuan;LIN Ming;ZHU Qing;ZHOU Juan;SUN G ui-Zhi;ZHAO Cui-Yun;NIE Xiang-Min;LIU Hong;CHEN Yuan-Feng;ZHU Chuan-Fu;Blood Center of Shandong Province;;Effects of N-Arachidonoylethanolamine on the Quality of Platelets Stored in M-sol Platelet Preservative Solution In Vitro[J];Journal of Experimental Hematology;2013-05
9 FU Yue;JIANG Jun;XU Wan-hua;LUO Yin-qiu;MAO Xiao-ying;TAN Jian-shao;HUANG Zi-tong;Department of Emergency Medicine ,The Affiliated Foshan Hospital of Sun Yat-sen University;Department of Emergency Medicine,Sun Yat-sen Memorial Hospital of Sun Yat-sen University;;Protective Effect of Cardiopulmonary Resuscitation on Brain Mitochondrial Function in a Rat Model of Cardiac Arrest[J];Journal of Sun Yat-sen University(Medical Sciences);2013-04
10 ZHENG Tian-sheng;LI Xiang;The First Clinical Medical College of Nanjing Medical University;Department of Orthopaedics,Nanjing Medical University First Affiliated Hospital;;Research Progress in Mitochondrial Apoptosis Pathway[J];Medical Recapitulate;2013-18
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved