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《Chinese Journal of Blood Transfusion》 2014-06
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The effects of N-Arachidonoylethanolamine on cytochrome C release and apoptosis of storage platelets

ZHANG Yunlong;LI Peng;LIU Yan;ZHANG Yi;QIAO Wenben;YU Yuan;FANG Yunhai;XU Qun;ZHU Chuanfu;ZHANG Wenjing.;Blood Center of Shandong Province;  
Objective To evaluate the effect of NArachidonoylethanolamine( ANA) on cytochrome C( Cyt-C)release and apoptosis in platelets( PLTs). Methods Apheresis PLTs taken from donors were split into two parts. An aliquot of0. 5 μmol /L ANA final concentration was added to one part of storage PLTs as the experimental group( n = 20),the other with-out ANA as the control group( n = 20). The two groups samples were stored on a flat-bed shaker at( 22 ± 2) ℃ up to 7days. The expression of phosphatidyl serine( PS) positive was determined by flow cytometer. The expressions of Cyt-C and caspase-3 were detected by Western blotting. And caspase-3 activity was measured by ELISA method. Results Comparing the experimental group with the control group,the rates of PLT PS positive were( 8. 29 ± 1. 44) % vs( 14. 24 ± 2. 47) %( P 0. 05); the expressions of activated caspase-3 were( 0. 063 ± 0. 014) % vs( 0. 132 ± 0. 025) %( P 0. 05); the activities of caspase-3 were 0. 096 ± 0. 04 vs 0. 208 ± 0. 03( P 0. 05); the releases of Cyt-C from mitochondria to cytosol were( 3. 18± 1. 32) % vs( 15. 13 ± 3. 40) %( P 0. 05). Conclusion ANA can inhibit cytochrome C release into the cytoplasm,and reduce caspase-3 activity and PLT apoptosis. This may be one important mechanism of protecting PLT from apoptosis by ANA inhibiting mitochondria-mediated pathway.
【Fund】: 山东省优秀中青年科学家科研奖励基金(BS2011SW044);; 山东省医药卫生科技发展计划重点项目(2013WS0170)
【CateGory Index】: R446.11
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