Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Blood Transfusion》 2014-06
Add to Favorite Get Latest Update

Detection of the copy numbers of human coagulation Factor Ⅶ in CHO cell by fluorescent quantitative polymerase chain reaction

XIAO Wei;LI Changqing;LIN Fangzhao;Institute of Blood Transfusion,Chinese Academy of Medical Sciences;  
Objective To detect the copy numbers of Human Coagulation Factor Ⅶ( FⅦ) gene in stable expression CHO cell line with fluorescent quantitative polymerase chain reaction( qPCR). Methods The quantitive genomic DNA of plasmid pMDT-actin and pcDNA-FⅦ were used as templates for making standard curves,respectively. The result was analyzed by Stepone software V2. 2 to calculate the total cell number and the copy number of FⅦ gene in templates. All of the qPCR reactions were performed in ABI Stepone plus system. Results The total cell number in templates is 24 421 ± 7 414.The copy number of the FⅦ gene is 49 455 ± 3 502. No specific gene amplication can be detected in the CHO-pcDNA3. 1.Conclusion The result clearly showed that every CHO cell harbored two FⅦ gene copies.
【CateGory Index】: R440
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 CHEN Xu, QI Fengkun, KANG Ligong, LI Jingfu(College of Horticulture, Northeast Agricultural University, Harbin 150030, China);Advance and application of real-time fluorescent quantitative PCR[J];Journal of Northeast Agricultural University;2010-08
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 DUAN Min et al(College of Life Sciences,Northwest A & F University,Yangling,Shaanxi 712100);Review of RQ-PCR Technology and Its Application in Wheat Genetic Testing[J];Journal of Anhui Agricultural Sciences;2012-10
2 LIN Fang1,FU Ruimin2,PI Chuanzhen3,SHI Kai3,YE Gang3,JIANG Jingyu3,CHEN Huizhen3(1.Department of Life Science and Biotechnology,Xinxiang College,Xinxiang He'nan 453000,China;2.Population and Life Department,Henan Institute of Education,Zhengzhou 450046,China;3.Banghe Pharmaceutical Co.Ltd,Zhengzhou 450001,China);Detecting trace host DNA based on SYBR Green I fluorescence method[J];Journal of Northeast Agricultural University;2011-07
3 Zhou Gang1,3,Qiu Dawei1,3,Qin Dajiang2,Niu Lizhi1,3,Cai Jinlei2,He Lihua1,3,Huang Wenhao2,Xu Kecheng1,3(1.The GIBH Affiliated Fuda Hospital,Chinese Academy of Sciences,Guangzhou 510310,China;2.StemCell Research Group,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences, Guangzhou 510610,China;3.Fuda Cancer Hospital of Guangzhou,Guangzhou 510305,China);Detection of the expression of CD44v6 gene in patients with pancreatic cancer by duplex real-time reverse transcription polymerase chain assay[J];International Journal of Laboratory Medicine;2011-18
4 MENG Lingbo1,2,LIU Guanjun1,3,LI Shumin4,XU Dandan2,QIN Zhiwei1(1.School of Horticulture,Northeast Agricultural University,Harbin 150030,China;2.Department of Life Science,Harbin University,Harbin 150086,China;3.The Laboratory of Forest Genetics and Breeding and Biotechnology of Ministry of Education,Northeast Forestry University,Harbin 150040,China;4.School of Resources and Environmenal Sciences,Northeast Agricultural University,Harbin 150030,China);Sequence analyses of non-specific transfer protein from cucumber and expression after challenging by Pseudomonas syringae pv.Lachrymans[J];Journal of Northeast Agricultural University;2013-01
5 LIU He;Centre for Animal Disease Prevention and Control in Fushun City;;Quality Control of RT-PCR in Veterinary Laboratory[J];Progress in Veterinary Medicine;2013-10
6 HAN Hui;ZHANG Jie;QI Jun-yao;TAN Ke-wei;WANG Jing-qi;XU Bao-liang;Chinese Academy of Inspection and Quarantine;;Advances on detection technology of Lyme disease[J];Chinese Journal of Frontier Health and Quarantine;2013-05
7 Xu Nana;Li Pengchang;Yu Jinfeng;Department of Plant Pathology,College of Plant Protection,Shandong Agricultural University;;Quantitative Detection of Rhizoctonia cerealis in Soil by Real-Time PCR[J];Shandong Agricultural Sciences;2014-03
8 SONG Xiao-bing;PENG Ai-tian;CHEN Xia;CHENG Bao-ping;LING Jin-feng;Research Institute of Plant Protection,Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of High Technology for Plant Protection;;Research advances of Citrus Huanglongbing pathogen culture and detection technology[J];Guangdong Agricultural Sciences;2013-23
9 WANG Xiao-nan;ZHANG Huan-rong;College of Life Science and Technology,Southwest University for Nationalities;Key Laboratory of Veterinary Medicine of University in Sichuan Province;;Proliferation of Infectious Bursal Disease Virus in Chicken Embryo Fibroblasts[J];Progress in Veterinary Medicine;2014-06
10 WANG Qing,LIN Hui-rong,ZHANG Shu-ting,YU Xin(Key Laboratory of Urban Environment and Health,Institute of Urban Environment,Chinese Academy of Sciences,Xiamen 361021,China);Real-time PCR Detection and Quantification of Emerging Waterborne Pathogens (EWPs) and Antibiotic Resistance Genes (ARGs) in the Downstream Area of Jiulong River[J];Environmental Science;2012-08
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Chao,MENG Xiangchen(Key Lab of Dairy Science,Ministry of Education,Food College,Northeast Agricultural University,Harbin 150030,China);Detection for Bifidobacterium by molecular beacon[J];Journal of Northeast Agricultural University;2008-03
2 AO Jinxia1, GAO Xuejun1, QIU Youwen1, GUO Shicheng2 (1. Life Sciences and Biotechnique Reseach Center, Northeast Agricultural University, Harbin 150030, China; 2. College of Life Sciences, Northeast Agricultural University, Harbin 150030, China);Real-time quantitative PCR and the application in the detection of genetically modified plants[J];Journal of Northeast Agricultural University;2009-06
3 Liu Lingfeng, Wang Kemin , Tan Weihong, Li Jun, Meng Xiangxian, Guo Qiuping, Tang Zhiwen, Liu Xuanming, Li Du (Institute of Biological Technology, College of Chemistry & Chemical Engineering, State Key Lab. of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082);A Novel Convenient and Fast Method for Detection of Tobacco Mosaic Virus-Ribonucleic Acid Based on Molecular Beacon[J];Chinese Journal of Analytical Chemistry;2003-09
4 QI Yanxiang1, XIAO Qiming1, ZHU Shuifang2 (1.College of Plant Protection, HNAU,Changsha410128,PRC; 2.Institute of Animal and Plant Quarantine, AQSIQ, Beijing100029,PRC);16S rDNA Gene Clone and Detection of Pantoea stewartii subsp.stewartii by Real-Time Fluorescent PCR[J];Journal of Hunan Agricultural University;2003-03
5 Qi yanxiang1 Xie yixian1 Zhang Huiqiang1 Zhu Shuifang2 Xiao Qiming3 Liao Xiaolan3 (1 Environment and Plant Protection Institute, CATAS, Danzhou, Hainan, 571737; 2 Institute of Animal and Plant Quarantine, AQSIQ, Beijing, 100029; 3 College of Bio-Safety Science And Technology, Hunan Agriculture University, Changsha, 410128);Detection of Ralstonia Solanacearum by Real-time Fluorescent PCR[J];Journal of South China University of Tropical Agriculture;2005-01
6 HUANG Guo-Ming~* LIAO Fang LIU Yue-Ting CUI Tie-Jun LUO Jia-Feng (Tianjin Entry-Exit Inspection and Quarantine Bureau,Tianjin 300456);Detection of Neotyphodium coenophialum and N.Iolii basedon real-time fluorescence PCR[J];Mycosystema;2007-02
7 WU Xing-hai,SHAO Xiu-ling,DENG Ming-jun,CHEN Chang-fa,LI Yan,LIANG Cheng-zhu (Shandong Exit-entry Inspection and Quarantine Bureau,Qingdao 266002,China);Study on Rapid Detection of Clavibacter michiganensis subsp. michiganensis by Real-time Fluorescent PCR[J];Acta Agriculturae Jiangxi;2007-03
8 LIAO Xiao-Lan1 ZHU Shui-Fang2** ZHAO Wen-Jun2 LUO Kuan1 QI Yan-Xiang1 CHEN Hong-Yun2 HE Kun1 ZHU Xiao-Xiang1( 1. College of Plant Protection, Hunan Agricultural University, Changsha 41012 8, China; 2. Animal and Plant Quarantine Institute, State Administration of Ent ry-exit Inspection and Quarantine of China, Beijing 100029, China);Cloning and Sequencing of Citrus Huanglongbing Pathogen 16S rDNA and Its Detection by Real-time Fluorescent PCR[J];Journal of Agricultural Biotechnology;2004-01
9 GUO Zhao-kui YAN Pei-qiang WAN Xiu-qing LI Li-jie YANG Qian(Heilongjiang Tobacco Research Institute Mudanjiang 157011, China) (Harbin Institute of Technology Department of Biotechnology Harbin 150001, China);Study on the Real-time PCR Quantity Detecting Methods of Genetic Modified Tobacco[J];Progress In Biotechnology;2005-01
10 GAO Qiang~(1,2) WU Pin-Shan~(1) ZHU Shui-Fang~(1) DAI Liang-Ying~(3) LIAO Hui-Min~2(Chinese Academy of Inspection and Quarantine,Beijing 100029)~(1)(College of Resource and Environment,Jishou University,Jishou 416000)~(2)(College of Plant Pretection,Hunan Agriculture University,Changsha 410128)~(3);Detection and Identification of Tilletia controversa Kühn by Real-time fluorescent PCR[J];Microbiology;2005-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved