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《Chinese Journal of Blood Transfusion》 2016-01
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Study on routes of infection and viral genotypes among HCV infected blood donors in Guangzhou

WANG Min;CHEN Jinyan;XU Ru;LIAO Qiao;LIANG Mingyue;XIONG Huaping;HUANG Jieting;HUANG Ke;SHAN Zhengang;RONG Xia;FU Yongshui;Institute of Blood Transfusion,Guangzhou Blood Center;  
Objective To study the routes of infection and viral genotypes / subtypes in volunteer blood donors infected with HCV in Guangzhou. Methods 296 samples which were reactive in ELISA test for anti-HCV were collected from individuals who donated blood voluntarily between October 2009 and July 2013 in Guangzhou and were successfully followed up.HCV RNA was tested by transcription-mediated amplification( TMA). Samples with undetectable HCV RNA levels were further confirmed for the presence of anti-HCV antibody using a third-generation recombinant immunoblot assay( RIBA). RNA were extracted from HCV RNA( +) samples by membrane absorption method and were subjected to detect for the presence of E1 and NS5 B by RT-PCR followed by Sanger sequencing. Sequence analysis and HCV genotyping were performed using DNASTAR,Bio Edit and Mega5. 0 softwares. Routes of infection were identified by questionnaire surveys. Results Different percentage of routes of infection among 197 confirmed HCV infected donors( 157 RNA positive and 40 RIBA positive)were as followed: intra venous drug use( IVDU) 8. 63%( 17 /197),transfusions of blood and / or blood products 17. 26%( 34 /197),other routes( such as surgery,tattoos,etc) 13. 71%( 27 /197),and unknown 60. 40%( 119 /197). The proportion in age were as followed: 18- 25 32. 49%( 64 /197),25- 35 26. 39%( 52 /197),35- 45 29. 95%( 59 /197),and 45- 54 11. 17%( 22 /197). Overall,wide age distribution and their routes of infection were significantly different( P 0. 05). 153 out of 157 HCV RNA( +) samples were successfully genotyped. The frequencies of subtypes HCV-1b,2a,3a,3b,6a were 58. 17%( 89 /153),4. 58%( 7 /153),7. 84%( 12 /153),3. 27%( 5 /153) and 26. 14 %( 40 /153),respectively. There were significant difference in HCV genotype distribution between intravenous drug users and blood donors who received transfusions of blood and / or blood products( P 0. 01). There was high frequency of HCV-6a among blood donors in Guangzhou: 58. 82%( 10 /17) were intra venous drug users. There was also high frequency of 1b: 54. 16%( 13 /24)were blood donors who received transfusions of blood and / or blood products. Conclusion There was a high proportion of HCV infection with unknown routes among blood donors in Guangzhou. HCV-1b and HCV-6a were the most predominant two subtypes among blood donors in Guangzhou. There was a high proportion of subtype 6a among intra venous drug users and 1b among blood donors who received transfusions of blood and / or blood products before.
【Fund】: 国家重大科技专项(2012ZX10004702);; 广州市医药卫生科技项目(2014A010060);; 广州市医学重点实验室项目(201509010009)
【CateGory Index】: R512.63;R440
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