Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Blood Transfusion》 2016-04
Add to Favorite Get Latest Update

Preliminary study on the improvement of the lesion of stored red blood cells by sodium pyruvate

XIA Sha;CHEN Gan;WANG Ying;YOU Guoxing;LIU Qingjun;ZHAO Lian;ZHOU Hong;Academy of Military Medical Science;  
Objective To investigate the effect of sodium pyruvate( SP) on red blood cells( RBCs) damage during storage. Methods The mice were anesthetized with intraperitoneal injections of sodium pentobarbital and mechanically ventilated. Mice were bled by cardiac puncture into CPDA-1 for anticoagulant and storage solution. The whole blood was leu-koreduced and divided into two groups equally( n = 8) : 1). SP group,where the concentrated solution of SP was added to RBC suspension to make the SP concentration 2. 5 mmol / L in a RBCs storage solution; 2) normal saline( NS) group,where RBCs suspension was added to saline at the same ratio as SP group,then was centrifuged at 400 g for 15 min. Its volume was reduced to a final hematocrit of 70% to 75%. The RBCs were stored at 4℃ for 14 days. After that,red blood cell counts were measured by blood cell automatic counter. The ATP level and MDA level were measured by ATP and MDA assay kits,respectively. Results After 14 days of storage,red blood cell counts in both of the two groups had no significant difference( P 0. 05),and all the indexes are normal. The ATP content of RBCs in NS group and SP group( nmol / mg protein) were 2. 46 ± 0. 19 vs 2. 82 ± 0. 21( P 0. 05). The MDA content in NS group and SP group( nmol / mg protein) were 15. 45 ± 0. 33 vs 14. 66 ± 0. 87( P 0. 05). Conclusion During RBC storage,according to the results of the ATP levels and MDA levels in two groups,the SP plays an important role in alleviating the lesion that occurred during storage.
【Fund】: 国家自然科学基金青年科学基金项目(81300751) 国家自然科学基金面上项目(31271001)
【CateGory Index】: R457.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 WANG Suling;WANG Qie;HE Tian-bo;HAN Wei;Department of Laboratory,Hebei Blood Center;Department of Anatomy,Hebei Medical University;Department of Laboratory,the Fourth Hospital of Hebei Medical University;;The effects of astaxanthin on the storage quality of suspended red blood cells[J];中国输血杂志;2015-03
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 XIA Sha;CHEN Gan;WANG Ying;YOU Guoxing;LIU Qingjun;ZHAO Lian;ZHOU Hong;Academy of Military Medical Science;;Preliminary study on the improvement of the lesion of stored red blood cells by sodium pyruvate[J];中国输血杂志;2016-04
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 ZHONG Rui;WANG Hong;ZHANGXI Dunzhu;HE Zeng;LIU Hua;CIDAN Pingcuo;HUANG Xiaoqian;WU Xia;ZHANG Jingdan;LIU Jiaxin;Institute of Blood Transfusion,Chinese Academy of Medical Sciences and Peking Union Medical College;Tibet Autonomous Region Blood Center;;The study of storage quality of suspended red blood cells in Tibetan plateau[J];中国输血杂志;2014-07
2 CHEN Jufen,HUANG Wenguang,WEN Chengrong,SHEN Chishi,YE Youwan,HUANG Yuanyuan. 1. Longgang Blood station,Shenzhen,Guangdong, 518172; 2. The Centers for Disease Control and Prevention of Pingshan,Shenzhen,Guangdong.;Effects of leukocyte depletion on hemorheologic properties of banked red blood cells[J];中国输血杂志;2013-07
3 LU Wen-yi,ZHAO Ming-feng,Sajin Rajbhandary,ZHAO Nan,XIE Fang,XIAO Xia,MU Juan,LI Yu-ming Department of Hematology,Tianjin First Central Hospital,the First Central Clinical College of Tianjin Medical University,Tianjin 300192,China;Effect and Mechanism of Iron-catalyzed Oxidative Stress on Mesenchymal Stem Cells[J];中国医学科学院学报;2013-01
4 CHEN Dong-fang,WANG Hai-yu,ZHANG Cong-ke,et al.(Henan Centre for Diseases Control and Prevention,Zhengzhou 450016,China);STUDY ON THE ANTIOXIDATION EFFECT OF CAPSULE OF NATURAL ASTAXANTHIN ON HUMAN BODY[J];现代预防医学;2011-04
Similar Journals
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved